Journal
BIOTECHNOLOGY ADVANCES
Volume 55, Issue -, Pages -Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.biotechadv.2021.107902
Keywords
Aptamer; Manipulating; SELEX; Post-SELX; Engineering
Categories
Funding
- National Natural Science Foundation of China [31871881]
- National First-Class Discipline Program of Food Science and Technology [JUFSTR20180303]
- Jiangsu Specially Appointed Professor Program
- National High-Level Personnel of Special Support Program [W03020371]
- Jiangsu Provincial Postdoctoral Research Funding Program [2021K305C]
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This article reviews the application potential of aptamers in biology and biomedicine, points out the limitations of aptamers at present, and proposes strategies to improve their performance.
Aptamers are short single-stranded DNA or RNA oligonucleotides isolated from randomized libraries, presenting high affinity and specificity toward targets with similar to antibodies. Recently, aptamers have demonstrated their outstanding application potential ranging from biosensors, bioimaging, and therapeutics due to various advantages including low immunogenicity, ease of large-scale synthesis, low batch-to-batch variation, easily chemical modification, and programmability. However, the current overall success rate of aptamer is far from being satisfactory, and still needs to overwhelm the major obstacle in affinity, specificity, and stability for practical application. The efficient strategy of manipulating the binding performance of aptamers, and improving their performance in practical application is of great significance. Herein, we review the challenges and advances in manipulating the binding performance (e.g., affinity, specificity, and stability) of aptamers to obtain high-quality aptamers by comprehensive analyzing their structure and properties. This mainly includes interfering with the SELEX protocol to obtain excellent parental aptamers, engineering aptamers in post-SELEX process and matching the optimal working microenvironment. Moreover, the perspectives of future direction are also summarized. We hope to provide researchers with alternative strategies for enhancing the performance of aptamers in practical applications and promoting the explosive development of aptamers in related fields.
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