Journal
BIOSENSORS & BIOELECTRONICS
Volume 200, Issue -, Pages -Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2021.113921
Keywords
Antifouling biosensor; Peptide; Electrochemical analysis; Gold nanorod; Prostate specific antigen
Categories
Funding
- National Natural Science Foundation of China [21705088]
- National Key Technology R&D Program of China [2017YFD0501500]
- Shandong Provincial Peanut Industry Tech-nology System Project [SDAIT-04-09]
- pharmaceutical scientific research guidance plan of Qingdao [2020-WJZD079, 2020-WJZD073]
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A highly sensitive biosensor with ultralow fouling properties was developed for the detection of prostate specific antigen (PSA) in complex biological media. The biosensor utilized specially designed antifouling peptides and a signal amplification strategy. The sensor exhibited a low limit of detection, a wide linear range, and could successfully detect PSA in real human serum, indicating its potential for practical application.
The development of biosensors capable of averting biofouling and detecting biomarkers in complex biological media remains a challenge. Herein, an ultralow fouling and highly sensitive biosensor based on specifically designed antifouling peptides and a signal amplification strategy was designed for prostate specific antigen (PSA) detection in human serum. A low fouling layer of poly(ethylene glycol) (PEG) doped the conducting polymer poly(3,4-ethylenedioxythiophene) (PEDOT) was electrodeposited on the electrode surface, followed by the immobilization of streptavidin and further attachment of biotin-labelled peptides. The peptide was designed to include PSA specific recognition domain (HSSKLQK) and antifouling domain (PPPPEKEKEKE), and the terminal of the peptide was functionalized with -SH group. DNA functionalized gold nanorods (DNA/AuNRs) were then attached to the electrode, and methylene blue (MB) molecules were adsorbed to the DNA to form the signal amplifier. In the presence of PSA, the peptide was specifically cleaved and resulted in the loss of AuNRs together with DNA and MB, and thus significant decrease of the current signal. The biosensor exhibited a low limit of detection (LOD) of 0.035 pg mL(-1) (S/N = 3), with a wide linear range from 0.10 pg mL(-1) to 10.0 ng mL(-1), and it was able to detect PSA in real human serum owing to the presence of the antifouling peptides, indicating great potential of the constructed biosensor for practical application.
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