Versatile potentiometric metabolite sensing without dioxygen interference

The field of electrochemical biosensors has been dominated by amperometric and voltammetric sensors; how -ever, these are limited greatly in their signal dependence on electrode size. Open circuit potentiometric sensors are emerging as an alternative due to their signal insensitivity to electrode size. Here, we present a second-generation biosensor that uses a modified chitosan hydrogel to entrap a dehydrogenase or other oxidoreductase enzyme of interest. The chitosan is modified with a desired electron mediator such that in the presence of the analyte, the enzyme will oxidize or reduce the mediator, thus altering the measured interfacial potential. Using the above design, we demonstrate a swift screening method for appropriate enzyme-mediator pairs based on open circuit potentiometry, as well as the efficacy of the biosensor design using two dehydrogenase enzymes (FADGDH and ADH) and peroxidase. Using 1,2-naphthoquinone as the mediator for FADGDH, dynamic ranges from 0.1 to 50 mM glucose are achieved. We additionally demonstrate the ease of fabrication and modification, a lifetime of >= 28 days, insensitivity to interferents, miniaturization to the microscale, and sensor efficacy in the presence of the enzyme's natural cofactor. These results forge a foundation for the generalized use of potentiometric biosensors for a wide variety of analytes within biologically-relevant systems where oxygen can be an interferent.

Automated summary

Open circuit potentiometric sensors are emerging as an alternative to traditional amperometric and voltammetric sensors due to their insensitivity to electrode size. This study presents a second-generation biosensor using a modified chitosan hydrogel to entrap enzymes and an electron mediator. The biosensor design allows for swift screening of enzyme-mediator pairs and demonstrates efficacy in glucose detection.