☆ 4.8 Article

Isothermal amplification-assisted diagnostics for COVID-19

BIOSENSORS & BIOELECTRONICS (2022)

Related references

Note: Only part of the references are listed.

Article Biophysics

Rapid and simultaneous visual screening of SARS-CoV-2 and influenza virufses with customized isothermal amplification integrated lateral flow strip

Yong Sun et al.

Summary: This work introduces a new detection technology that can simultaneously test for SARS-CoV-2 and influenza viruses, with advantages such as speed, high sensitivity, no cross-reactivity, and false positives. Its clinical application demonstrates high accuracy, and this portable device is expected to meet the global healthcare system's need for online monitoring of serious infectious diseases.

BIOSENSORS & BIOELECTRONICS (2022)

Add to Collection

Article Biophysics

A Palm Germ-Radar (PaGeR) for rapid and simple COVID-19 detection by reverse transcription loop-mediated isothermal amplification (RT-LAMP)

Anle Ge et al.

Summary: The study developed a device called PaGeR for rapid and simple detection of COVID-19. The device uses four simple steps to achieve rapid detection of SARS-CoV-2, and can detect the virus selectively with naked-eye. It supports multiple detection methods including colorimetric, fluorometric and lateral dipstick readout. Experimental results with clinical RNA samples showed that the instrument can effectively diagnose COVID-19 patients.

BIOSENSORS & BIOELECTRONICS (2022)

Add to Collection

Review Public, Environmental & Occupational Health

Systematic review with meta-analysis of the accuracy of diagnostic tests for COVID-19

Beatriz Boger et al.

Summary: This study systematically reviewed the accuracy parameters of different diagnostic methods for detecting SARS-CoV-2, finding that computed tomography has high sensitivity but low specificity, while the combination of IgM and IgG antibodies showed promising results. The research also indicated that RT-PCR remains the gold standard for diagnosing COVID-19 in sputum samples, but combining different diagnostic methods can improve accuracy.

AMERICAN JOURNAL OF INFECTION CONTROL (2021)

Add to Collection

Review Chemistry, Analytical

State of the Art on the SARS-CoV-2 Toolkit for Antigen Detection: One Year Later

Laura Fabiani et al.

Summary: The recent global events of COVID-19 have highlighted the importance of rapid virus detection in order to prevent spread. Efforts have been made to develop new, rapid, affordable, and accurate diagnostic tools. Researchers have focused on electrochemical and optical biosensors for specific detection of SARS-CoV-2 antigens.

BIOSENSORS-BASEL (2021)

Add to Collection

Review Microbiology

Considerations for diagnostic COVID-19 tests

Olivier Vandenberg et al.

Summary: In this review, Vandenberg et al. explore the crucial role of diagnostic tests during the early phase of the COVID-19 pandemic, addressing technical and implementation issues encountered and suggesting future directions for improved diagnostics in potential future outbreaks.

NATURE REVIEWS MICROBIOLOGY (2021)

Add to Collection

Article Chemistry, Analytical

Suitcase Lab for Rapid Detection of SARS-CoV-2 Based on Recombinase Polymerase Amplification Assay

Ahmed Abd El Wahed et al.

Summary: A rapid point-of-need detection method for SARS-CoV-2 was developed based on RT-RPA assay, which can detect the virus in approximately 15 minutes. The method was validated in three different laboratories in Africa, showing high accuracy and potential for assisting in detecting COVID-19 cases in low-resource settings to speed up control measures.

ANALYTICAL CHEMISTRY (2021)

Add to Collection

Article Biophysics

Accessible detection of SARS-CoV-2 through molecular nanostructures and automated microfluidics

Haitao Zhao et al.

Summary: The integrated platform, named eSIREN, utilizes responsive enzyme-DNA nanostructures and automated microfluidics to directly detect SARS-CoV-2 RNA targets near patients. The technology offers advantages of rapid, accurate, and easy operation, with a detection limit of 7 copies of RNA target per µL and completion in <20 min at room temperature.

BIOSENSORS & BIOELECTRONICS (2021)

Add to Collection

Article Multidisciplinary Sciences

Development and comparison of novel multiple cross displacement amplification (MCDA) assays with other nucleic acid amplification methods for SARS-CoV-2 detection

Laurence Don Wai Luu et al.

Summary: The development of alternative isothermal amplification assays, such as multiple cross displacement amplification (MCDA), may overcome the speed and portability limitations of real-time PCR (rt-PCR) methods for SARS-CoV-2 detection. MCDA was found to be the fastest nucleic acid amplification method for SARS-CoV-2, while rt-PCR exhibited the highest sensitivity. Different nucleic acid amplification methods offer distinct advantages, which should be taken into consideration when selecting the most suitable method for specific applications.

SCIENTIFIC REPORTS (2021)

Add to Collection

Article Chemistry, Multidisciplinary

Handheld Point-of-Care System for Rapid Detection of SARS-CoV-2 Extracted RNA in under 20 min

Jesus Rodriguez-Manzano et al.

Summary: The study presents a rapid PoC diagnostic test based on RT-LAMP and semiconductor technology for detecting SARS-CoV-2 in less than 20 minutes. The test demonstrates high sensitivity and specificity in clinical samples, showing promise as a rapid detection tool for tackling the COVID-19 pandemic.

ACS CENTRAL SCIENCE (2021)

Add to Collection

Article Chemistry, Analytical

Reverse Transcription Recombinase Polymerase Amplification Coupled with CRISPR-Cas12a for Facile and Highly Sensitive Colorimetric SARS-CoV-2 Detection

Wei S. Zhang et al.

Summary: The proposed method combines RT-RPA with CRISPR-Cas12a colorimetric assay for SARS-CoV-2 detection, utilizing DNA-modified gold nanoparticles to achieve high sensitivity and specificity. This novel approach can effectively avoid false positive results from other beta coronavirus members, improving the practical testing availability of SARS-CoV-2.

ANALYTICAL CHEMISTRY (2021)

Add to Collection

Review Biophysics

Diagnosis of COVID-19 for controlling the pandemic: A review of the state-of-the-art

Nastaran Taleghani et al.

Summary: Health organizations and countries globally are struggling to control the spread of COVID-19, with scientists working on rapid testing and vaccine development. Fast and accurate diagnosis is crucial for disease prevention and control, and ongoing research and technology advancements are key in this effort.

BIOSENSORS & BIOELECTRONICS (2021)

Add to Collection

Article Biochemical Research Methods

Isothermal recombinase polymerase amplification-lateral flow detection of SARS-CoV-2, the etiological agent of COVID-19

Thomas R. Shelite et al.

Summary: The rapid development of an RPA-LF testing method has shown successful detection of SARS-CoV-2, with high accuracy and potential for use in resource-limited and decentralized laboratories.

JOURNAL OF VIROLOGICAL METHODS (2021)

Add to Collection

Article Immunology

S-Variant SARS-CoV-2 Lineage B1.1.7 Is Associated With Significantly Higher Viral Load in Samples Tested by TaqPath Polymerase Chain Reaction

Michael Kidd et al.

Summary: Analysis of recent test data shows that samples containing the SARS-CoV-2 variant B1.1.7 with mutation Delta 69/70 exhibit S gene target failure (SGTF) in ThermoFisher TaqPath RT-qPCR. Samples with SGTF profile are more likely to have higher viral loads, indicating higher infectivity and rapid spread of this variant.

JOURNAL OF INFECTIOUS DISEASES (2021)

Add to Collection

Article Multidisciplinary Sciences

Rapid electrochemical detection of coronavirus SARS-CoV-2

Thanyarat Chaibun et al.

Summary: The researchers developed an ultrasensitive electrochemical biosensor based on isothermal rolling circle amplification for rapid detection of SARS-CoV-2, capable of detecting as low as 1 copy/mu L of N and S genes in clinical samples. The sensor showed a 100% concordance with qRT-PCR results in clinical sample evaluation.

NATURE COMMUNICATIONS (2021)

Add to Collection

Article Chemistry, Analytical

Combined recombinase polymerase amplification/rkDNA-graphene oxide probing system for detection of SARS-CoV-2

Moon Hyeok Choi et al.

Summary: The study combined RPA with an rkDNA-GO probe system for rapid and highly sensitive detection of SARS-CoV-2, exhibiting high selectivity and sensitivity. The total processing time was only 1.6 hours, making it an efficient method for point-of-care detection of SARS-CoV-2.

ANALYTICA CHIMICA ACTA (2021)

Add to Collection

Article Biophysics

Harnessing recombinase polymerase amplification for rapid multi-gene detection of SARS-CoV-2 in resource-limited settings

Dounia Cherkaoui et al.

Summary: The study introduces a rapid, simple, highly sensitive, and multiplexed molecular detection method which simultaneously targets two conserved genes, offering potential advantages and application prospects. The technology demonstrates high specificity for detecting various coronaviruses and shows promise for point-of-care testing.

BIOSENSORS & BIOELECTRONICS (2021)

Add to Collection

Article Biophysics

PfAgo-based detection of SARS-CoV-2

Fei Wang et al.

Summary: In this study, a highly sensitive and accurate molecular diagnosis platform named SARS-CoV-2 PAND was developed for large-scale screening of COVID-19 infection. The use of Pyrococcus furiosus Argonaute technology allowed for rapid detection within an hour, significantly reducing the time needed for Real-time fluorescence quantitative PCR instrument usage. This platform showed 100% consistency with RT-qPCR in diagnosing clinic samples.

BIOSENSORS & BIOELECTRONICS (2021)

Add to Collection

Article Biophysics

Sequence-specific and multiplex detection of COVID-19 virus (SARS-CoV-2) using proofreading enzyme-mediated probe cleavage coupled with isothermal amplification

Sheng Ding et al.

Summary: A new RT-Proofman-LAMP detection method has been developed in this study, utilizing proofreading enzyme-mediated probe cleavage to specifically detect SARS-CoV-2 with real-time and visual detection capabilities. This method offers versatility and high specificity for fast field screening and laboratory testing of COVID-19, making it a promising platform in the diagnosis of COVID-19.

BIOSENSORS & BIOELECTRONICS (2021)

Add to Collection

Article Biology

Engineered RNA biosensors enable ultrasensitive SARS-CoV-2 detection in a simple color and luminescence assay

Anirudh Chakravarthy et al.

Summary: This research presents a highly sensitive diagnostic assay for COVID-19 and its variants using toehold RNA-based sensors. By optimizing RNA amplification and biosensor design, the assay is capable of detecting viral RNA as low as 100 copies with bright color development, easily visualized and quantifiable by human eye and spectrophotometry.

LIFE SCIENCE ALLIANCE (2021)

Add to Collection

Editorial Material Medicine, General & Internal

SARS-CoV-2 Variants in Patients with Immunosuppression

Lawrence Corey et al.

NEW ENGLAND JOURNAL OF MEDICINE (2021)

Add to Collection

Review Microbiology

The Emerging Concern and Interest SARS-CoV-2 Variants

Edyta Janik et al.

Summary: Since the discovery of the SARS-CoV-2 responsible for COVID-19 in December 2019, the virus has continually mutated, with three variants of concern and four variants of interest being distinguished in recent months and requiring close monitoring.

PATHOGENS (2021)

Add to Collection

Article Chemistry, Analytical

Padlock probe-based rolling circle amplification lateral flow assay for point-of-need nucleic acid detection

Sidhartha Jain et al.

Summary: The sensitive nucleic acid detection method based on padlock probe rolling circle amplification, nuclease protection, and lateral flow detection has been developed for use in resource-limited settings. By combining these techniques, the method allows for detection of antimicrobial resistance genes and specific pathogens such as SARS-CoV-2 with high sensitivity.

ANALYST (2021)

Add to Collection

Article Biochemical Research Methods

A microfluidic-integrated lateral flow recombinase polymerase amplification (MI-IF-RPA) assay for rapid COVID-19 detection

Dan Liu et al.

Summary: The MI-IF-RPA assay developed in this study offers a rapid and sensitive detection of SARS-CoV-2, integrating RT-RPA and LF detection systems into a single microfluidic chip. The assay provides easily interpretable results with a short testing time of approximately 30 minutes, showing high sensitivity and specificity, making it a cost-effective screening tool for resource-limited areas in complement to RT-PCR.

LAB ON A CHIP (2021)

Add to Collection

Article Multidisciplinary Sciences

A new coronavirus associated with human respiratory disease in China

Fan Wu et al.

NATURE (2020)

Add to Collection

Article Virology

Evaluation of rapid diagnosis of novel coronavirus disease (COVID-19) using loop-mediated isothermal amplification

Yutaro Kitagawa et al.

JOURNAL OF CLINICAL VIROLOGY (2020)

Add to Collection

Article Multidisciplinary Sciences

Insights on early mutational events in SARS-CoV-2 virus reveal founder effects across geographical regions

Carlos Farkas et al.

PEERJ (2020)

Add to Collection

Article Medicine, General & Internal

Early Phylogenetic Diversification of SARS-CoV-2: Determination of Variants and the Effect on Epidemiology, Immunology, and Diagnostics

Rene Kaden

JOURNAL OF CLINICAL MEDICINE (2020)

Add to Collection

Article Infectious Diseases

Rapid and visual detection of 2019 novel coronavirus (SARS-CoV-2) by a reverse transcription loop-mediated isothermal amplification assay

C. Yan et al.

CLINICAL MICROBIOLOGY AND INFECTION (2020)

Add to Collection

Article Public, Environmental & Occupational Health

Variant analysis of SARS-CoV-2 genomes

Takahiko Koyama et al.

BULLETIN OF THE WORLD HEALTH ORGANIZATION (2020)

Add to Collection

Article Biochemistry & Molecular Biology

A Rapid, Simple, Inexpensive, and Mobile Colorimetric Assay COVID-19-LAMP for Mass On-Site Screening of COVID-19

Franklin Wang-Ngai Chow et al.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2020)

Add to Collection

Review Biology

Loop-Mediated Isothermal Amplification (LAMP): A Rapid, Sensitive, Specific, and Cost-Effective Point-of-Care Test for Coronaviruses in the Context of COVID-19 Pandemic

Robin Augustine et al.

BIOLOGY-BASEL (2020)

Add to Collection

Article Pathology

Development of Reverse Transcription Loop-Mediated Isothermal Amplification Assays Targeting Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)

Gun-Soo Park et al.

JOURNAL OF MOLECULAR DIAGNOSTICS (2020)

Add to Collection

Article Biophysics

The impact of biosensing in a pandemic outbreak: COVID-19

Eden Morales-Narvaez et al.

BIOSENSORS & BIOELECTRONICS (2020)

Add to Collection

Article Biophysics

Homogeneous circle-to-circle amplification for real-time optomagnetic detection of SARS-CoV-2 RdRp coding sequence

Bo Tian et al.

BIOSENSORS & BIOELECTRONICS (2020)

Add to Collection

Article Biophysics

Multiplex reverse transcription loop-mediated isothermal amplification combined with nanoparticle-based lateral flow biosensor for the diagnosis of COVID-19

Xiong Zhu et al.

BIOSENSORS & BIOELECTRONICS (2020)

Add to Collection

Article Respiratory System

Highly sensitive and specific diagnosis of COVID-19 by reverse transcription multiple cross-displacement amplification-labelled nanoparticles biosensor

Shijun Li et al.

EUROPEAN RESPIRATORY JOURNAL (2020)

Add to Collection

Article Infectious Diseases

SARS-CoV-2 samples may escape detection because of a single point mutation in the N gene

Katharina Ziegler et al.

EUROSURVEILLANCE (2020)

Add to Collection

Article Virology

Artificial Intelligence-Assisted Loop Mediated Isothermal Amplification (AI-LAMP) for Rapid Detection of SARS-CoV-2

Mohammed A. Rohaim et al.

VIRUSES-BASEL (2020)

Add to Collection

Article Virology

Use of the variplex™ SARS-CoV-2 RT-LAMP as a rapid molecular assay to complement RT-PCR for COVID-19 diagnosis

Juergen Roedel et al.

JOURNAL OF CLINICAL VIROLOGY (2020)

Add to Collection

Article Biotechnology & Applied Microbiology

Mutations on COVID-19 diagnostic targets

Rui Wang et al.

GENOMICS (2020)

Add to Collection

Article Chemistry, Analytical

Detection of Biomarkers in Blood Using Liquid Crystals Assisted with Aptamer-Target Recognition Triggered in Situ Rolling Circle Amplification on Magnetic Beads

Lubin Qi et al.

ANALYTICAL CHEMISTRY (2019)

Add to Collection

Review Chemistry, Analytical

Recombinase polymerase amplification: Basics, applications and recent advances

Ivan Magrina Lobato et al.

TRAC-TRENDS IN ANALYTICAL CHEMISTRY (2018)

Add to Collection

Review Biophysics

Advances in isothermal amplification: novel strategies inspired by biological processes

Jia Li et al.

BIOSENSORS & BIOELECTRONICS (2015)

Add to Collection

Article Biochemistry & Molecular Biology

Oligonucleotide gap-fill ligation for mutation detection and sequencing in situ

Marco Mignardi et al.

NUCLEIC ACIDS RESEARCH (2015)

Add to Collection

Article Multidisciplinary Sciences

Rapid and Sensitive Isothermal Detection of Nucleic-acid Sequence by Multiple Cross Displacement Amplification

Yi Wang et al.

SCIENTIFIC REPORTS (2015)

Add to Collection

Article Biochemical Research Methods

Comparison of conventional RT-PCR, reverse-transcription loop-mediated isothermal amplification, and SYBR green I-based real-time RT-PCR in the rapid detection of bovine viral diarrhea virus nucleotide in contaminated commercial bovine sera batches

Shu-Qin Zhang et al.

JOURNAL OF VIROLOGICAL METHODS (2014)

Add to Collection

Article Biochemical Research Methods

LAVA: An Open-Source Approach To Designing LAMP (Loop-Mediated Isothermal Amplification) DNA Signatures

Clinton Torres et al.

BMC BIOINFORMATICS (2011)

Add to Collection

Review Toxicology

Molecular techniques for the study and diagnosis of parasite infection

R. G. Tavares et al.

JOURNAL OF VENOMOUS ANIMALS AND TOXINS INCLUDING TROPICAL DISEASES (2011)

Add to Collection

Article Biotechnology & Applied Microbiology

Quantification of the detrimental effect of a single primer-template mismatch by real-time PCR using the 16S rRNA gene as an example

D. Bru et al.

APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2008)

Add to Collection

Article Biochemical Research Methods

Loop-mediated isothermal amplification (LAMP) of gene sequences and simple visual detection of products

Norihiro Tomita et al.

NATURE PROTOCOLS (2008)

Add to Collection

Article Chemistry, Analytical

Detection of six sing le-nucleotide polymorphisms associated with rheumatoid arthritis by a loop-mediated isothermal amplification method and an electrochemical DNA chip

Naoko Nakamura et al.

ANALYTICAL CHEMISTRY (2007)

Add to Collection

Article Biochemistry & Molecular Biology

DNA detection using recombination proteins

Olaf Piepenburg et al.

PLOS BIOLOGY (2006)

Add to Collection

Article Chemistry, Multidisciplinary

Rolling-circle amplification of a DNA nanojunction

Chenxiang Lin et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2006)

Add to Collection

Review Biochemistry & Molecular Biology

Nucleic acid aptamers and enzymes as sensors

Naveen K. Navani et al.

CURRENT OPINION IN CHEMICAL BIOLOGY (2006)

Add to Collection

Review Biochemical Research Methods

Multiplexed protein profiling on antibody-based microarrays by rolling circle amplification

SF Kingsmore et al.

CURRENT OPINION IN BIOTECHNOLOGY (2003)

Add to Collection

Article Biochemistry & Molecular Biology

L-RCA (ligation-rolling circle amplification): a general method for genotyping of shingle nucleotide polymorphisms (SNPs)

XQ Qi et al.

NUCLEIC ACIDS RESEARCH (2001)

Add to Collection

© Peeref 2019-2024. All rights reserved.