Isothermal amplification-assisted diagnostics for COVID-19

The scenery of molecular diagnostics for infectious diseases is rapidly evolving to respond to the COVID-19 epidemic. The sensitivity and specificity of diagnostics, along with speed and accuracy, are crucial requirements for effective analytical tools to address the disease spreading around the world. Emerging diagnostic devices combine the latest trends in isothermal amplification methods for nucleic acids with state-of-the-art biosensing systems, intending to bypass roadblocks encountered in the last 2 years of the pandemic. Isothermal nucleic acid amplification is a simple procedure that quickly and efficiently accumulates nucleic acid sequences at a constant temperature, without the need for sophisticated equipment. The integration of isothermal amplification into portable biosensing devices confers high sensitivity and improves screening at the point of need in low-resource settings. This review reports the latest trends reached in this field with the latest examples of isothermal amplification-powered biosensors for detecting SARS-CoV-2, with different configurations, as well as their intrinsic advantages and disadvantages.

Automated summary

The landscape of molecular diagnostics for infectious diseases is evolving rapidly in response to the COVID-19 epidemic. A combination of isothermal nucleic acid amplification methods and biosensing systems is being employed to enhance sensitivity and efficiency in nucleic acid detection, addressing the spread of the disease. This technology allows for rapid accumulation of nucleic acid sequences at a constant temperature without sophisticated equipment, making it suitable for point-of-need screening in low-resource settings.