Journal
BIOSENSORS & BIOELECTRONICS
Volume 200, Issue -, Pages -Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2021.113925
Keywords
SARS-CoV-2; COVID-19; RT-LAMP; Rapid detection; Palm germ-radar
Categories
Funding
- National Key Research & Development Program [2017YFF0204601]
- National Natu-ral Science Foundation of China [31800084, 21775155]
- Scientific Research Project of General Administration of Customs, CHINA [2020HK134]
- Natural Science Foundation of Shandong Province [ZR2018ZC0128, ZR2019BC017]
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The study developed a device called PaGeR for rapid and simple detection of COVID-19. The device uses four simple steps to achieve rapid detection of SARS-CoV-2, and can detect the virus selectively with naked-eye. It supports multiple detection methods including colorimetric, fluorometric and lateral dipstick readout. Experimental results with clinical RNA samples showed that the instrument can effectively diagnose COVID-19 patients.
The current COVID-19 pandemic caused by SARS-CoV-2 is raging, seriously threatening people's lives. The establishment of rapid and accurate pathogen detection technology is not only critical in this epidemic, but also a reminder that we must always be prepared for possible future outbreaks. Therefore, we developed a Palm GermRadar (PaGeR) device for rapid and simple detection of COVID-19 from extracted patient sample RNA by RTLAMP. The whole procedure of rapid COVID-19 detection is based on 4 simple steps: inactivation, extraction, amplification, and detection. SARS-CoV-2 down to 1 copy/mu L could be detected selectively with naked-eye. Three detection methods (colorimetric, fluorometric and lateral dipstick readout) could be performed in PaGeR instrument. By employing the PaGeR, we successfully detected SARS-CoV-2 in clinical RNA samples isolated from swab specimens. The results showed that 15 out of 17 COVID-19 patients were diagnosed as positive while all 55 normal samples were diagnosed as negative. Therefore, the developed PaGeR instrument can realize the detection of COVID-19 with easily visualized results, providing a promising instrument for rapid detection in the community as well as at home.
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