Degradation mechanism of montmorillonite-enhanced antibiotic wastewater: performance, antibiotic resistance genes, microbial communities, and functional metabolism

The effective degradation of Sulfamethoxazole (SMX) is of great importance to alleviate environmental pollution. In this study, the degradation capacity of an ordinary sequencing batch activated sludge system (SBR) and montmorillonite (MMT) system was compared for their ability to degrade different concentrations of SMX. Compared with SBR system, the MMT system exhibited higher stability and degradation capacity. The changes in the composition of tightly bound extracellular polymeric substances (TB-EPS) were likely key to the observed stability of the system. High concentrations of SMX inhibited the degradation performance of SBR. MMTsupplemented reduced the generation of antibiotic resistance genes (ARGs). Thauera is a gene that is able to degrade SMX, and its abundance in MMT system reached 7.84%. As potential hosts of ARGs, the proportions of Paenarthrobacter and Caldilineacea were significantly correlated with sulfonamide resistance genes (sul1 and sul2). Overall, MMT-supplemented system was found to be a favorable method of treating antibiotic.

Automated summary

This study compared the degradation capacity of an ordinary SBR system and an MMT system for different concentrations of SMX. The results showed that the MMT system exhibited higher stability and degradation capacity compared to the SBR system. The changes in the composition of TB-EPS were likely key to the observed stability of the MMT system.