4.8 Article

Microbial production of medium-chain-length α, ω-diols via two-stage process under mild conditions

Journal

BIORESOURCE TECHNOLOGY
Volume 352, Issue -, Pages -

Publisher

ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2022.127111

Keywords

Medium-chain-length diols; Monooxygenase; Esterase; Pseudomonas putida; Biocatalyst

Funding

  1. Chinese Scholarship Council

Ask authors/readers for more resources

This study developed a biocatalyst for the production of mcl-diols from n-alkanes under mild conditions. The engineered strain P. putida KT2440, with overexpression of Est12, can efficiently hydrolyze esterified diols, enabling the production of mcl-diols.
Medium-chain-length alpha, omega-diols (mcl-diols) are versatile compounds widely used as building blocks of coating materials and polymers. Mcl-diols are currently synthesized through energy intensive chemical process. Recently, esterified diols have been produced from n-alkanes in E. coli by co-expression of the alkane monooxygenase module (AlkBGTL) and the esterification module (Atf1), thereby establishing the technical feasibility of the process. However, esterified diols need to be hydrolyzed for further applications. In this study, we developed bio-catalysts for mcl-diol production from n-alkanes under mild conditions. The engineered P. putida KT2440 with overexpression of Est12 can efficiently hydrolyze esterified diols (C6-C10). Later, the engineered strain was co-cultured with an E. coli strain (AlkBGTL-Atf1) to produce mcl-diols. In a two-stage approach, 5 mM 1,6-hexane-diol was produced, 61.5 times of one-stage test, from n-hexane by biocatalysts for the first time. In conclusion, the present work indicates that bio-catalysis offers a green biobased alternative for synthesis of mcl-diols.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.8
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available