Enhancing enzymatic hydrolysis efficiency of crop straws via tetrahydrofuran/water co-solvent pretreatment

Pretreatment and enzymatic hydrolysis are critical steps in bio-ethanol production from lignocellulose. The enhancement of enzymatic hydrolysis of several typical crop straws and the particle size adaptability of sorghum straw by tetrahydrofuran/water co-solvent pretreatment were studied. Efficient cellulose conversions (>83.2%) and adequate hemicellulose conversion (>40.7%) were obtained from pretreated rice straw, sorghum straw, wheat straw and corn stover in enzymatic hydrolysis, and the highest glucose yield and xylose yield were 274.0 and 26.3 mg/g dry solid, respectively. Glucose production of 140.4 mg/mL was obtained when the pretreated sorghum straw with mixed particle sizes was employed in enzymatic hydrolysis at 20% solid loading (8 FPU/g cellulose). When the enzyme loading reduced to 4 FPU/g cellulose, 221.7 mg/g dry solid glucose yield and 68.6% enzymatic hydrolysis efficiency could be still obtained with 15% solid loading, exhibiting high potential for bioethanol production.

Automated summary

Tetrahydrofuran/water co-solvent pretreatment enhances enzymatic hydrolysis of various crop straws and enables sorghum straw to adapt to different particle sizes. The pretreated sorghum straw shows potential for bioethanol production with high glucose yield and enzymatic hydrolysis efficiency.