The relationship between material transformation, microbial community and amino acids and alkaloid metabolites in the mushroom residue-prickly ash seed oil meal composting with biocontrol agent addition

This study investigated the effects of adding biocontrol microbes on metabolites and pathogenic microorganisms during mushroom residue composting and the relationships of metabolite changes with microbes and material transformation. The results showed that the addition of Bacillus subtilis (BS) and Trichoderma harzianum (TH) with mushroom residue promoted the conversion of organic carbon and nitrogen. The abundance of pathogenic microbes was increased in biocontrol microbial treatments. BS or TH treatments increased the levels of amino acids, carbohydrates, and bacteriostatic alkaloid metabolites. Network analysis revealed that the main micro -organisms significantly related to alkaloid metabolites were Rhabdanaerobium, Atopostipes, Planifilum and Ure-ibacillus. The increased bacterial abundance and decreased NO3--N and TOC were closely related to the increases in amino acid and alkaloid metabolites after biocontrol agent treatments. Generally, adding biocontrol microbes is an effective way to increase the levels of antibacterial metabolites, but there is a risk of increasing the abundance of pathogenic microbes.

Automated summary

This study investigated the effects of adding biocontrol microbes on metabolites and pathogenic microorganisms during mushroom residue composting. The results showed that adding Bacillus subtilis and Trichoderma harzianum promoted the conversion of organic carbon and nitrogen. The treatments with these microbes increased the levels of amino acids, carbohydrates, and bacteriostatic alkaloid metabolites. The increased bacterial abundance was closely related to the increase in amino acid and alkaloid metabolites after biocontrol agent treatments.