Insight into halotolerance of a robust heterotrophic nitrifying and aerobic denitrifying bacterium Halomonas salifodinae

Studies toward biotreating hypersaline wastewater containing different salts and halotolerant mechanism of robust strains are important but still rare. Here an isolated bacterium Halomonas salifodinae can perform simultaneous nitrification and denitrification (SND) at 15% salinity, showing high nitrogen removal efficiencies of over 98% via response surface methodology optimization. Besides NaCl, this robust strain had high resistance to other salts (KCl, Na2SO4, and K2SO4) and can efficiently remove nitrogen in saline wastewater containing heavy metals such as Fe(II), Mn(II), Zn(II), Cr(VI), Ni(II), and Cu(II). After repeated-batch culturing at different salinities, the treated strains with different halotolerant capabilities were used as single strain model to study halotolerant mechanism via metabolic analysis. The halotolerant bacterium can convert D-proline and glutamic acid to glutamine as well as lactulose to trehalose. The accumulated intracellular compatible solutes can resist high osmotic pressure and bound water molecule in hypersaline wastewater to accomplish high-efficiency SND processes.

Automated summary

This study investigates the ability of a halophilic bacterium to perform simultaneous nitrification and denitrification in hypersaline wastewater, as well as its halotolerant mechanism. The results show that the isolated strain can efficiently remove nitrogen at 15% salinity and exhibits high resistance to various salts and heavy metals. Metabolic analysis reveals that the bacterium accumulates compatible solutes to resist high osmotic pressure and bound water molecules, achieving efficient nitrification and denitrification processes.