Vertical-flow constructed wetland based on pyrite intensification: Mixotrophic denitrification performance and mechanism

Deep nitrogen removal from low-carbon wastewater is a pressing water treatment challenge as of yet. Eight sets of vertical-flow constructed wetland (VFCW) intensified by pyrite were designed and applied to treat with low C/ N ratio wastewater in this research. The results showed that the addition of pyrite (100% added) significantly promoted TN removal with an efficiency higher than 27.05% under low C/N ratio conditions, indicating that mixotrophic denitrification was achieved in VFCW. Microbial analysis showed that the community structure and diversity of microorganisms were changed significantly, and the growth of autotrophic (Thiobacillus) and heterotrophic bacteria (Thauera) concomitantly enhanced. It is recommended that the addition amount of pyrite is 75% of the wetland volume, meantime, mixing evenly with 25% high porosity substrate (such as activated carbon, volcanic stone, etc.), which could enhance the effective adhesion of microorganisms and their contact area with pyrite, ultimately improve the denitrification capacity of the VFCW.

Automated summary

In this study, eight sets of VFCWs were designed to treat low C/N ratio wastewater, and the addition of pyrite significantly improved the denitrification efficiency. Microbial analysis revealed changes in microbial community structure and diversity, as well as enhanced growth of autotrophic and heterotrophic bacteria, contributing to improved denitrification capacity.