Journal
BIORESOURCE TECHNOLOGY
Volume 353, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2022.127127
Keywords
Acetate; C1 gas; Poly-3-hydroxybutyrate; Metabolic engineering; Methylorubrum extorquens AM1
Funding
- C1 Gas Refinery Program through the National Research Foundation of Korea (NRF) - Ministry of Science and ICT [2015M3D3A1A01064919, 2018M3D3A1A01055733]
- National Research Foundation of Korea [2018M3D3A1A01055733] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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This study successfully improved the production of bioplastic poly-3-hydroxybutyrate (PHB) using acetate as the sole carbon source through metabolic engineering and the supply of energy cofactors. The yields of biomass and PHB from acetate significantly improved, and the growth rate and PHB content of the bacteria increased. The sustainability of PHB production was demonstrated by using acetate derived from carbon dioxide and syngas.
Acetate is regarded as a sustainable microbial feedstock that is synthesized from biowastes such as synthesis gas (syngas), carbon dioxide, lignocellulose, or organic waste. In this study, Methylorubrum extorquens AM1 was engineered to improve the production of bioplastic poly-3-hydroxybutyrate (PHB) using acetate as the sole carbon source. To utilize acetate as a carbon source and methanol as an energy source, acs encoding acetyl-CoA synthetase and fdh from Burkholderia stabilis were overexpressed, while ftfL involved in the assimilation of methanol into formyl-tetrahydrofolate was deleted. The yields of biomass and PHB from acetate significantly improved, and the growth rate and PHB content of the bacteria increased. In addition, sustainability of the PHB production was demonstrated using acetate derived from carbon dioxide and syngas. This study shows that biopolymers could be synthesized efficiently using acetate as the sole carbon source through metabolic engineering and the supply of energy cofactors.
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