Effect of magnetite supplementation on mesophilic anaerobic digestion of phenol and benzoate: Methane production rate and microbial communities

Anaerobic sequential batch tests treating phenol and benzoate were conducted to evaluate the potential of magnetite supplementation to improve methanogenic degradation of phenol and benzoate, and to identify active microbial communities under each condition. Specific CH4 production rates during anaerobic digestion were 218.5 mL CH4 /g VSS/d on phenol and 517.6 mL CH4 /g VSS/d on benzoate. Magnetite supplementation significantly increased methanogenic degradation of phenol by 9.0-68.0% in CH4 production rate, and decreased lag time by 7.9-48.0%, with no significant reduction in CH4 yield. Syntrophorhabdus, Sporotomaculum, Syntrophus, Syntrophomonas, Peptoclostridium, Soehngenia, Mesotoga, Geobacter, Methanosaeta, Methanoculleus, and Methanospirillum were revealed as active microbial communities involved in anaerobic digestion of phenol and benzoate. Magnetite-mediated direct interspecies electron transfer between Geobacter, Peptoclostridium, and Methanosaeta harundinacea could contribute to this improvement.

Automated summary

This study found that magnetite supplementation can significantly improve methanogenic degradation of phenol and benzoate, increasing methane production rates and reducing lag time, without reducing methane yield. Active microbial communities involved in the anaerobic digestion of phenol and benzoate were also identified, including Geobacter, Peptoclostridium, and Methanosaeta harundinacea.