Biodegradation of ceftriaxone in soil using dioxygenase-producing genetically engineered Pseudomonas putida

In this study, the degradability of the antibiotic Ceftriaxone was investigated with the help of genetically engineered Pseudomonas putida, in which the gene producing the enzyme catechol 2 and 3 dioxygenase was designed and then inserted into the pUC18 plasmid and replicated by E. coli. It was purified and extracted and transformed into Pseudomonas putida. Finally, the degradation rate of Ceftriaxone by this bacterium in spiked soil was evaluated using the HPLC measurement technique. Finally, the kinetics of Ceftriaxone degradation by genetically engineered Pseudomonas putida was investigated using zero, first, and second -order kinetic models for all factors. The results of HPLC measurement showed that the biodegradation of ceftriaxone in spiked soil was significant by genetically engineered P. putida compared to autoclaved soil inoculated by wild P. putida and normal soil with normal microbial flora (p < 0.001) and this bacterium was able to degrade ceftriaxone by 69.53% and kinetic modeling showed that the rate of removal by genetically engineered Pseudomonas putida follows the zero-degree reaction model. These findings indicate that Pseudomonas putida, which produces Catechol 2,3-dioxygenase, can be useful and practical in the biological treatment of environment from cephalosporins.

Automated summary

In this study, the degradability of the antibiotic Ceftriaxone was investigated using genetically engineered Pseudomonas putida. The results showed that the engineered Pseudomonas putida was able to significantly degrade Ceftriaxone, with a degradation rate of 69.53%. Kinetic modeling indicated that the rate of removal by the engineered Pseudomonas putida followed the zero-order reaction model. These findings suggest that Pseudomonas putida, which produces Catechol 2,3-dioxygenase, has potential practical applications in the biological treatment of cephalosporins in the environment.