Journal
BIOMATERIALS
Volume 285, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2022.121538
Keywords
Composite scaffold; Bone regeneration; Glycopeptide hydrogel; Macrophage polarization; Immunomodulation
Funding
- National Natural Science Foundation of China [51661010, 31971306, 32171380]
- Natural Sci-ence Fund for Distinguished Young Scholars of Tianjin [21JCJQJC00020]
- Fundamental Research Funds for the Central Universities [2019PT350005]
- CAMS Innovation Fund for Medical Sciences [2021-I2M-1-065, 2021-I2M-1-058]
- Tianjin Innova-tion and Promotion Plan Key Innovation Team of Implantable and Interventional Biomedical Materials
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A synthetic fibrous glycopeptide hydrogel was developed and composited with 3D-printed PCL/nHA scaffold for cranial bone regeneration, showing significant promotion of stem cell proliferation and osteogenic differentiation as well as enhanced interaction between macrophages and stem cells. The composite scaffold exhibited superior efficacy in bone regeneration and osseointegration, creating a reparative microenvironment similar to normal cranium with increased anti-inflammatory macrophages and osteoblasts.
The reconstruction of large cranial bone defects by bioactive materials without exogenous cells or growth factors remains a substantial clinical challenge. Here, synthetic fibrous glycopeptide hydrogel (GR(gel)) self-assembled by beta-sheet RADA16-grafted glucomannan was designed to mimic the glycoprotein composition and the fibrillar architecture of natural extracellular matrix (ECM), which was non-covalently composited with 3D-printed polycaprolactone/nano hydroxyapatite (PCL/nHA) scaffold for cranial bone regeneration. The glycopeptide hydrogel significantly promoted the proliferation, osteogenic differentiation of bone mesenchymal stem cells (BMSCs), which was further augmented by GR(gel)-induced macrophage M2-phonotype polarization and the effective M2 macrophage-BMSC crosstalk. The repair of critical-size skull bone defect in rat indicated a superior efficacy of PCL/nHA@GR(gel) implant on bone regeneration and osseointegration, with an average bone area of 83.3% throughout the defect location at 12 weeks post treatment. Furthermore, the osteo-immunomodulatory GR(gel) induced a reparative microenvironment similar with that in normal cranium, as characterized by an increased percentage of anti-inflammatory M2 macrophages and osteoblasts, and high-level vascularization. Collectively, the composite scaffold developed here with macrophage polarization-mediated osteo-immunomodulation may represent a promising implant for expediting in situ bone regeneration by providing biochemical and osteoinductive cues at the injured tissue.
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