Journal
BIOMARKERS
Volume 27, Issue 6, Pages 587-598Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/1354750X.2022.2077446
Keywords
Alzheimer's disease; transgene; Drosophila melanogaster; Solanum spp
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Funding
- International Foundation for Science (IFS)
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The leaves of African eggplant and Black nightshade improved the behavioral and biochemical indices in a Drosophila model of Alzheimer's disease through neural enzyme modulation and antioxidant mechanisms.
Introduction We investigated the effect of African eggplant (AE) (Solanum macrocarpon L) and Black nightshade (BN) (Solanum nigrum L) leaves; two tropical vegetables consumed by humans on behavioural, biochemical and histological indices in Drosophila melanogaster model of Alzheimer's disease (AD). Materials and Method Transgenic flies expressing human Amyloid Precursor Protein (hAPP) and beta-secretase (hBACE 1) were exposed to the pulverised leaf samples (0.1 and 1.0%) in their diets for fourteen days. Thereafter, the flies were assessed for their behavioural indices and routine histology of brain cells. Furthermore, fly head homogenates were assayed for beta-amyloid level, activities of acetylcholinesterase (AChE) and beta-secretase (BACE-1), as well as oxidative stress markers. Results Result showed that the significantly lower (p < 0.05) behavioural parameters (survival, locomotor performance and memory index), higher AChE and BACE-1 activities, beta-amyloid, ROS and lipid peroxidation levels, as well as reduced antioxidant indices observed in the AD flies, were significantly ameliorated (p < 0.05) in AD flies treated with the leaf samples. Discussion This study has showed that leaves of AE and BN ameliorated behavioural and biochemical indices in AD flies via neural enzyme modulatory, and antioxidant mechanisms Conclusion Hence, this study further justifies the neuroprotective properties of both AE and BN.
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