Journal
BIOELECTROCHEMISTRY
Volume 144, Issue -, Pages -Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.bioelechem.2021.108038
Keywords
Trimethylamine N-oxide; Electrochemically Active Bacteria; Shewanella loihica PV-4; Extracellular Electron Transfer; Bioelectrochemical System; Biosensor
Funding
- Beijing Institute of Technology Research Fund Program for Young Scholars [3160011182123]
- China Postdoctoral Science Foundation [2020 M670099]
- Fundamental Research Funds for the Central Universities
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A novel TMAO detection method based on microbial electrochemical technology was established in this study, enabling direct conversion of TMAO concentration into electrical signals. The method showed a wide linear range, high sensitivity, low detection limit, and achieved 90% accuracy within 600 seconds in real serum samples.
Trimethylamine N-oxide (TMAO) is considered to be a novel biomarker of cardiovascular diseases. However, the traditional TMAO detection method has failed to meet the requirements of real-time and point-of-care tests. Herein, a novel TMAO detection method based on microbial electrochemical technology is established, which realizes the direct conversion of TMAO concentration into electrical signals. Attached Shewanella loihica PV-4 was first proven to be capable of simultaneous inward extracellular electron transfer and TMAO reduction. The TMAO detection method showed a wide linear range of 0 to 250 mu M, a high sensitivity of 23.92 mu A/mM, and a low limit of detection of 5.96 mu M. In addition, the TMAO detection process was accomplished within 600 s, with an acceptable accuracy of 90% in the real serum, showing high feasibility in clinical applications. (C) 2021 Elsevier B.V. All rights reserved.
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