4.4 Article

Importance of Asparagine 202 in Manipulating Active Site Structure and Substrate Preference for Human CYP17A1

Related references

Note: Only part of the references are listed.
Article Biochemistry & Molecular Biology

Mechanism of the Clinically Relevant E305G Mutation in Human P450 CYP17A1

Yilin Liu et al.

Summary: This study discusses the mechanisms of steroid metabolism in humans, including the role of cytochrome P450, the impact of mutations on metabolism, and the mechanism of bond cleavage reactions. The research reveals that specific mutations can alter enzyme activity, substrate binding modes, and product selectivity.

BIOCHEMISTRY (2021)

Article Chemistry, Multidisciplinary

Substrate-Specific Allosteric Effects on the Enhancement of CYP17A1 Lyase Efficiency by Cytochrome b5

Yilin Liu et al.

Summary: This study used resonance Raman spectroscopy to compare the effect of Mn-substituted cytochrome b(5) on the oxy complex of CYP17A1, resulting in a specific allosteric interpretation for the more pronounced acceleration seen with the 17OH-PROG substrate. The presence of Mn-b led to a reorientation of a hydrogen bond between the 17OH group of the substrate and the proximal oxygen atom, favoring lyase catalysis, but this effect was not observed for 17OH-pregnenolone.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2021)

Article Chemistry, Multidisciplinary

P450 CYP17A1 Variant with a Disordered Proton Shuttle Assembly Retains Peroxo-Mediated Lyase Efficiency

Yilin Liu et al.

CHEMISTRY-A EUROPEAN JOURNAL (2020)

Article Biochemistry & Molecular Biology

Human P450 CYP17A1: Control of Substrate Preference by Asparagine 202

Michael C. Gregory et al.

BIOCHEMISTRY (2018)

Review Chemistry, Multidisciplinary

Formation and Cleavage of C-C Bonds by Enzymatic Oxidation Reduction Reactions

F. Peter Guengerich et al.

CHEMICAL REVIEWS (2018)

Article Chemistry, Multidisciplinary

Human Cytochrome CYP17A1: The Structural Basis for Compromised Lyase Activity with 17-Hydroxyprogesterone

Piotr J. Mak et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2018)

Article Biochemistry & Molecular Biology

Investigating the roles of T224 and T232 in the oxidation of cinnamaldehyde catalyzed by myxobacterial CYP260B1

Martin Litzenburger et al.

FEBS LETTERS (2017)

Article Chemistry, Medicinal

Mechanism of Cytochrome P450 17A1-Catalyzed Hydroxylase and Lyase Reactions

Silvia Bonomo et al.

JOURNAL OF CHEMICAL INFORMATION AND MODELING (2017)

Review Biochemistry & Molecular Biology

Steroid 17-hydroxylase and 17,20-lyase deficiencies, genetic and pharmacologic

Richard J. Auchus

JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY (2017)

Article Cell Biology

Structural insights into the function of steroidogenic cytochrome P450 17A1

Rahul Yadav et al.

MOLECULAR AND CELLULAR ENDOCRINOLOGY (2017)

Review Biochemistry & Molecular Biology

The role of CYP17A1 in prostate cancer development: structure, function, mechanism of action, genetic variations and its inhibition

Monika Kmet'ova Sivonova et al.

GENERAL PHYSIOLOGY AND BIOPHYSICS (2017)

Article Peripheral Vascular Disease

CYP17A1 Enzyme Activity Is Linked to Ambulatory Blood Pressure in a Family-Based Population Study

Daniel Ackermann et al.

AMERICAN JOURNAL OF HYPERTENSION (2016)

Article Multidisciplinary Sciences

Promising Tools in Prostate Cancer Research: Selective Non-Steroidal Cytochrome P450 17A1 Inhibitors

Silvia Bonomo et al.

SCIENTIFIC REPORTS (2016)

Article Multidisciplinary Sciences

Unveiling the crucial intermediates in androgen production

Piotr J. Mak et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2015)

Article Biochemistry & Molecular Biology

Active site proton delivery and the lyase activity of human CYP17A1

Yogan Khatri et al.

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2014)

Article Chemistry, Multidisciplinary

Differential Hydrogen Bonding in Human CYP17 Dictates Hydroxylation versus Lyase Chemistry

Michael Gregory et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2013)

Review Chemistry, Inorganic & Nuclear

CO, NO and O2 as vibrational probes of heme protein interactions

Thomas G. Spiro et al.

COORDINATION CHEMISTRY REVIEWS (2013)

Article Chemistry, Multidisciplinary

Kinetic Solvent Isotope Effect in Human P450 CYP17A1-Mediated Androgen Formation: Evidence for a Reactive Peroxoanion Intermediate

Michael C. Gregory et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2013)

Article Multidisciplinary Sciences

Structures of cytochrome P450 17A1 with prostate cancer drugs abiraterone and TOK-001

Natasha M. DeVore et al.

NATURE (2012)

Review Biochemistry & Molecular Biology

At the crossroads of steroid hormone biosynthesis: The role, substrate specificity and evolutionary development of CYP17

Andrei A. Gilep et al.

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS (2011)

Review Biochemistry & Molecular Biology

A review of mechanistic studies on aromatase (CYP19) and 17α-hydroxylase-17,20-lyase (CYP17)

Muhammad Akhtar et al.

JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY (2011)

Review Pharmacology & Pharmacy

Targeting the androgen receptor pathway in prostate cancer

Yu Chen et al.

CURRENT OPINION IN PHARMACOLOGY (2008)

Article Chemistry, Physical

Resonance Raman Characterization of the Peroxo and Hydroperoxo Intermediates in Cytochrome P450

Ilia G. Denisov et al.

JOURNAL OF PHYSICAL CHEMISTRY A (2008)

Article Biochemistry & Molecular Biology

Alteration of P450 distal pocket solvent leads to impaired proton delivery and changes in heme geometry

Thomas M. Makris et al.

BIOCHEMISTRY (2007)

Article Chemistry, Multidisciplinary

Resonance Raman detection of the hydroperoxo intermediate in the cytochrome P450 enzymatic cycle

Piotr J. Mak et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2007)

Article Chemistry, Multidisciplinary

Kinetic isotope effects on the rate-limiting step of heme oxygenase catalysis indicate concerted proton transfer/heme hydroxylation

R Davydov et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2003)

Article Biochemistry & Molecular Biology

CYP17 mutation E305G causes isolated 17,20-lyase deficiency by selectively altering substrate binding

DP Sherbet et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2003)

Article Biochemical Research Methods

High-level expression in Escherichia coli and purification of the membrane-bound form of cytochrome b5

SB Mulrooney et al.

PROTEIN EXPRESSION AND PURIFICATION (2000)