Journal
ADVANCED DRUG DELIVERY REVIEWS
Volume 89, Issue -, Pages 57-70Publisher
ELSEVIER
DOI: 10.1016/j.addr.2015.06.011
Keywords
Raman scattering; Inelastic light scattering; Single cell analysis; Cell physiology; Tissue physiology; Coherent Raman scattering; Label-free optical microscopy
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Funding
- NSF Center for Biophotonics Science and Technology
- NSF Science and Technology Center
- Div Of Industrial Innovation & Partnersh
- Directorate For Engineering [1343479] Funding Source: National Science Foundation
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Raman micro-spectroscopy provides a convenient non-destructive and location-specific means of probing cellular physiology and tissue physiology at sub-micron length scales. By probing the vibrational signature of molecules and molecular groups, the distribution and metabolic products of small molecules that cannot be labeled with fluorescent dyes can be analyzed. This method works well for molecular concentrations in the micro-molar range and has been demonstrated as a valuable tool for monitoring drug-cell interactions. If the small molecule of interest does not contain groups that would allow for a discrimination against cytoplasmic background signals, labeling of the molecule by isotope substitution or by incorporating other unique small groups, e.g. alkynes provides a stable signal even for time-lapse imaging such compounds in living cells. In this review we highlight recent progress in assessing the physiology of cells and tissue by Raman spectroscopy and imaging. (C) 2015 Elsevier B.V. All rights reserved.
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