Journal
ARCHIVES OF PATHOLOGY & LABORATORY MEDICINE
Volume 146, Issue 7, Pages 814-821Publisher
COLL AMER PATHOLOGISTS
DOI: 10.5858/arpa.2022-0041-SA
Keywords
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Funding
- National Natural Science Foundation of China [81971147, 81771312]
- Xiamen Science and Technology Planning Project [3502Z20209032]
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There is a strong correlation between neutralizing antibodies and anti-receptor-binding domain (RBD) IgG antibodies, suggesting that anti-RBD IgG antibody levels can accurately assess immunity following SARS-CoV-2 infection or vaccination.
Context.-Neutralizing antibody detection can assess the incidence of COVID-19 and the effectiveness of vaccines. However, commercial reagents for neutralizing antibodies were developed after the anti-SARS-CoV-2 immunoglobulin (Ig) G and IgM antibodies. Therefore, some laboratories did not perform neutralizing antibody testing services because of multiple factors. Objective.-To find a fast, accurate, and economic alternative for the detection of neutralizing antibodies for the development of COVID-19 screening programs. Design.-The response and correlation of 3 antibodies (anti-spike protein neutralizing antibody, total anti-receptor-binding domain [RBI)] antibody, and anti-RBD IgG) were determined by observing the dynamics in 61 participants for 160 days after vaccination. Results.-The levels of neutralizing and anti-RBD IgG antibodies reached their peak values on day 42 after vaccination (120.75 IU/mL and 14.38 signal-to-cutoff ratio [S/CO], respectively). The total antibody levels peaked at 138.47 S/CO on day 35 after vaccination. The strongest correlation was found between neutralizing and anti-RBD IgG antibody levels (r = 0.894, P < .001). The area under the receiver operating characteristic curve for total antibody levels for the prediction of seropositivity for neutralizing antibodies was 0.881 (P < .001), and that for anti-RBD IgG antibody levels was 0.937 (P < .001). Conclusions.-Neutralizing and anti-RBD IgG antibody levels were strongly correlated, and thus anti-RBD IgG antibody levels can be used for the accurate assessment of immunity following SARS-CoV-2 infection or vaccination.
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