Shinella oryzae sp. nov., a novel zearalenone-resistant bacterium isolated from rice paddy soil

A novel bacterium, designated Z-25( T), was isolated from a rice paddy rhizosphere soil sample from Wuchang County, China. The Z-25( T) strain is gram-negative, rod-shaped, non-spore-forming, aerobic, motile by unipolar flagella and straw white in color. A phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain Z-25 belongs to the genus Shinella, and the closest members are Shinella zoogloeoides ATCC 19623( T) with 98.58% similarity, S. kummerowiae CCBAU 25,048 T (98.03%) and S. granuli Ch06 (T) (97.37%). The average nucleotide identity and in silico DNA-DNA hybridization values between strain Z-25( T) and the closest members were less than 85.29% and 28.70%, respectively. The predominant fatty acids were the sums of features comprising C-18:1 omega 7c and/or C-18:1 omega 6c (34.62%), C-18:1 omega 7c -11-methyl (20.48%), and C-19:0 cyclo omega 8c (18.19%). The only respiratory quinone was ubiquinone-10, and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Additionally, a genome analysis showed that Z-25( T) presented potential functional genes related to the degradation of zearalenone (ZEN). An HPLC analysis indicated that Z-25( T) could remove 74.13% of 10 mg/L ZEN after 144 h at 30 degrees C. Therefore, based on phenotypic, chemotaxonomic, phylogenetic and genotypic analyses, strain Z-25( T) represents a novel species in the genus Shinella, for which the name Shinella oryzae sp. nov. is proposed. The type strain is Z-25( T) (= GDMCC 1.2424( T) = KCTC 82660( T)).

Automated summary

A novel bacterium, named Z-25(T), belonging to the genus Shinella and the new species Shinella oryzae sp. nov., was isolated from a rice paddy rhizosphere soil sample in Wuchang County, China. The strain showed potential for the degradation of ZEN.