Journal
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
Volume 115, Issue 5, Pages 661-695Publisher
SPRINGER
DOI: 10.1007/s10482-022-01720-0
Keywords
Schizosaccharomyces; Isolation method; Enrichment; Ecology
Categories
Funding
- Deutsche Bundesstiftung Umwelt DBU (German Federal Environmental Foundation) [34053/01-32]
- Ministry of Science and Technology of the People's Republic of China
- Beijing municipal government
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This study investigates the distribution and ecology of fission yeast species, including S. pombe, in various substrates. The results show that S. pombe and S. octosporus primarily inhabit honeybee honey, while S. osmophilus is mainly found in solitary bee beebread. S. japonicus is mainly found in forest substrates.
The fission yeast genus Schizosaccharomyces contains important model organisms for biological research. In particular, S. pombe is a widely used model eukaryote. So far little is known about the natural and artificial habitats of species in this genus. Finding out where S. pombe and other fission yeast species occur and how they live in their habitats can promote better understanding of their biology. Here we investigate in which substrates S. pombe, S. octosporus, S. osmophilus and S. japonicus are present. To this end about 2100 samples consisting of soil, tree sap fluxes, fresh fruit, dried fruit, honey, cacao beans, molasses and other substrates were analyzed. Effective isolation methods that allow efficient isolation of the above mentioned species were developed. Based on the frequency of isolating different fission yeast species in various substrates and on extensive literature survey, conclusions are drawn on their ecology. The results suggest that the primary habitat of S. pombe and S. octosporus is honeybee honey. Both species were also frequently detected on certain dried fruit like raisins, mango or pineapple to which they could be brought by the honey bees during ripening or during drying. While S. pombe was regularly isolated from grape mash and from fermented raw cacao beans S. octosporus was never isolated from fresh fruit. The main habitat of S. osmophilus seems to be solitary bee beebread. It was rarely isolated from raisins. S. japonicus was mainly found in forest substrates although it occurs on fruit and in fruit fermentations, too.
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