Journal
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
Volume 115, Issue 4, Pages 545-559Publisher
SPRINGER
DOI: 10.1007/s10482-022-01709-9
Keywords
Ectoine; Halomonas; Salt-induced osmoadaptation; Whole genome sequencing
Categories
Funding
- National Natural Science Foundation of China [31860030, 21967018]
- Natural Science Foundation of Qinghai, China [2018-ZJ-930Q]
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In this study, we reported the whole genome of a novel Halomonas species strain XH36 with high ectoine production potential. The analysis showed that XH36 belongs to a novel Halomonas species named Halomonas qaidamensis. Ectoine was the main compatible solute in XH36 and it accumulated ectoine to cope with salt stress. Our findings suggest that XH36 is a promising industrial strain for ectoine production and the genomic analysis provides insights into its salt-induced osmoadaptation mechanisms.
Here, we report the whole genome of a novel halophilic Halomonas species strain XH36 with high ectoine production potential. The genome was 3,818,310 bp in size with a GC content of 51.97%, and contained 3533 genes, 61 tRNAs and 18 rRNAs. The phylogenetic analysis using the 16s rRNA genes, the UBCGs and the TYGS database indicated that XH36 belongs to a novel Halomonas species, which we named as Halomonas qaidamensis. Osmoadaptation related genes including Na(+) and K(+) transport and compatible solute accumulation were both present in the XH36 genome, the latter of which mainly contained ectoine, 5-hydroxyectoine and betaine. HPLC validation studies showed that H. qaidamensis XH36 accumulated ectoine to cope with salt stress, and the content of ectoine could be as high as 315 mg/g CDW under 3 mol/l NaCl. Our results show that XH36 is a new promising industrial strain for ectoine production, and the genomic analysis will guide us to better understand its salt-induced osmoadaptation mechanisms, and provide theoretical references for future application research of ectoine.
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