Evidence of successive negative contrast in terrestrial toads (Rhinella arenarum): central or peripheral effect?

Prior research with terrestrial toads (Rhinella arenarum) in a water-reinforced instrumental situation indicated a direct relationship between acquisition rate and reward magnitude. However, a reward downshift produced a gradual adjustment of instrumental performance and a rapid adjustment of consummatory performance, rather than the abrupt and transient deterioration of behavior typical of a successive negative contrast effect. In Experiment 1, using a two-chamber box, a downshift from deionized water (which supports maximal rehydration) to 250-mM sodium chloride solution (which supports a lower rehydration), also yielded a gradual adjustment of instrumental behavior. In this experiment, animals received one trial per day and were allowed 300 s of access to the reward in the goal box. Experiment 2 used the same procedure, except that animals were allowed access to the solution in the goal box for 600 s. Under these conditions, reward downshift led to longer latencies (instrumental) and lower rehydration levels (consummatory) than those of unshifted controls, providing evidence for successive negative contrast. Unlike in similar experiments with mammals, the effect was not transient, but persisted relatively unmodified over twelve daily postshift trials. In this case, the possibility of adaptation of the peripheral mechanisms for water uptake is considered. The comparative relevance of these results is discussed in terms of habit formation versus expectancy-guided behavior in vertebrate learning.

Automated summary

Research on terrestrial toads (Rhinella arenarum) in a water-reinforced instrumental situation showed that a reward downshift led to gradual adjustment of instrumental performance and consummatory performance, different from the typical transient deterioration of behavior seen in a successive negative contrast effect. Evidence of successive negative contrast was found when animals were allowed longer access to the reward, with the effect persisting relatively unchanged over multiple daily trials. The results suggest a different response in toads compared to mammals and raise questions about adaptation mechanisms for water uptake in vertebrates.