CRISPR/cas9 cassette targeting Escherichia coli blaCTX-M specific gene of mastitis cow milk origin can alter the antibiotic resistant phenotype for cefotaxime

CTX-M beta-lactamases are one of the most important extended spectrum beta-lactamase (ESBL) resistance enzymes found in E. coli. In the present study, 59% of E. coli isolates from mastitis cow milk were reported to be positive for ESBL types. The prevalence of beta-lactam (beta-lactam) antibiotic resistance was reported to be 84%, 72.7%, 52.27%, 50%, and 45.4% for cefotaxime, cefepime, cefuroxime, oxacillin, and cephalexine, respectively. The (CTX)-C-bla-M gene was found in 65% (n = 17) of the E. coli isolates when they were genotyped. Further, the use of a CRISPR/cas9 cassette to target the E. coli (CTX)-C-bla-M gene revealed changes in antibiotic phenotypes for cefotaxime.

Automated summary

CTX-M beta-lactamases are important resistance enzymes in E. coli, and a high prevalence of ESBL-positive E. coli isolates was found in mastitis cow milk. The resistance rates to various beta-lactam antibiotics were also high, and targeting the (CTX)-C-bla-M gene using CRISPR/Cas9 technology revealed changes in antibiotic susceptibility.