Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 61, Issue 29, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202204098
Keywords
Droplet Microfluidics; Mass Spectrometry; Single Cells; Whole-Cell Catalysis; Microreactors
Categories
Funding
- Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) [FOR 2177, 251124697]
- Projekt DEAL
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Chemically specific and quantifiable monitoring of biocatalytic transformations at the single-cell level has been achieved through the integration of droplet microfluidics, cell imaging, and mass spectrometry.
Improving the performance of chemical transformations catalysed by microbial biocatalysts requires a deep understanding of cellular processes. While the cellular heterogeneity of cellular characteristics, such as the concentration of high abundant cellular content, is well studied, little is known about the reactivity of individual cells and its impact on the chemical identity, quantity, and purity of excreted products. Biocatalytic transformations were monitored chemically specific and quantifiable at the single-cell level by integrating droplet microfluidics, cell imaging, and mass spectrometry. Product formation rates for individual Saccharomyces cerevisiae cells were obtained by i) incubating nanolitre-sized droplets for product accumulation in microfluidic devices, ii) an imaging setup to determine the number of cells in the droplets, and iii) electrospray ionisation mass spectrometry for reading the chemical contents of individual droplets. These findings now enable the study of whole-cell biocatalysis at single-cell resolution.
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