Journal
ANALYTICAL LETTERS
Volume 55, Issue 17, Pages 2803-2816Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/00032719.2022.2072857
Keywords
Animal-derived food; cephalexin; high-performance liquid chromatography-mass spectrometry; indirect competitive enzyme-linked immunosorbent assay; monoclonal antibody
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Funding
- Key Research and Development Programof Hubei Province (CN) [2020BBB079]
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In this study, a specific anti-cephalexin monoclonal antibody was used to develop an ic-ELISA method for the determination of cephalexin in animal-derived foods. The established method showed high sensitivity and accuracy, and correlated well with HPLC-MS/MS analysis.
Cephalexin (CFE) residues in animal-derived food and its deleterious effects on human health has attracted extensive attention. In this study, a specific anti-cephalexin monoclonal antibody (mAb) was used to develop an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) with a half-maximal inhibitory concentration (IC50) of 1.83 mu g/L that was suitable for the determination of cephalexin in animal-derived foods. The limit of detection (LOD) and limit of quantitation (LOQ) for cephalexin were from 0.44 to 0.58 mu g/kg and 0.57 to 0.67 mu g/kg, respectively. The recovery was from 90.1 to 103.6% with coefficients of variation (CV) less than 10.0%. In addition, the established ELISA method and high-performance liquid chromatography - mass spectrometry (HPLC-MS/MS) were used to analyze the samples with good correlation between the results. The results show that the established ic-ELISA is suitable for the determination of cephalexin in animal-derived food.
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