Sensitive Fluorescent Determination of Chloramphenicol Based upon Graphdiyne and RecJf Exonuclease-Assisted Signal Amplification

A sensitive and selective method for determining chloramphenicol (CAP) based on RecJ(f) exonuclease-assisted signal amplification and graphdiyne (GDY) is reported. GDY possesses a strong absorptivity for single-stranded DNA and is proposed for the first time as a new specific and eminently sensitive type of fluorescence sensing platform for chloramphenicol. Under the optimized conditions, the assay operates from 10 to 80 mu g/L chloramphenicol with a 43.6 ng/L detection limit. The procedure was employed to determine chloramphenicol in food samples, with recoveries from 93.60% to 107.90%. The proposed technique offers a novel development in food safety using sensitive aptasensors for detecting small molecules.

Automated summary

The method combines RecJ(f) exonuclease with graphdiyne to achieve highly sensitive and selective detection of chloramphenicol, which is of great significance for the detection of chloramphenicol in food samples.