4.8 Article

Alternative Assay Reagents for UV-Spectroscopic Detection of (Pyro-)Phosphate with the PUB Module

Journal

ANALYTICAL CHEMISTRY
Volume 94, Issue 23, Pages 8132-8135

Publisher

AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.2c01404

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Funding

  1. Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) [492196858]
  2. Konrad-Adenauer Stiftung (KAS, Konrad-Adenauer Foundation)

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The report advises against using 5-iodoridine or 5-ethynyluridine as alternative assay reagents in the PUB module due to their lack of an isosbestic point of phosphorolysis under moderately alkaline conditions.
This report advises against the use of 5-iodoridine or 5-ethynyluridine as alternative assay reagents in the PUB module, primarily due to their lack of an isosbestic point of phosphorolysis under moderately alkaline conditions.

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