In Vitro/In Vivo Electrochemical Detection of Pt(II) Species

The biodistribution of chemotherapy compounds within tumor tissue is one of the main challenges in the development of antineoplastic drugs, and techniques for simple, inexpensive, sensitive, and selective detection of various analytes intumors are of great importance. In this paper we propose the use of platinized carbon nanoelectrodes (PtNEs) for the electrochemical detection of platinum-based drugs in various biological models, including single cells and tumor spheroidsin vitroand inside solidtumorsin vivo. We have demonstrated the quantitative directdetection of Pt(II) in breast adenocarcinoma MCF-7 cells treated with cisplatin and a cisplatin-based DNP prodrug. To realize thepotential of this technique in advanced tumor models, we measured Pt(II) in 3D tumor spheroidsin vitroand in tumor-bearing micein vivo. The concentration gradient of Pt(II) species correlated with the distance from the sample surface in MCF-7 tumor spheroids. We then performed the detection of Pt(II) species in tumor-bearing mice treated intravenously with cisplatin and DNP. We found that there was deeper penetration of DNP in comparison to cisplatin. This research demonstrates a minimally invasive, real-time electrochemical technique for the study of platinum-based drugs.

Automated summary

This paper introduces a technique for the detection of chemotherapy drugs using platinized carbon nanoelectrodes. Through studies on various biological models, it is found that this technique has the potential to be applied in advanced tumor models.