Journal
ANALYTICAL CHEMISTRY
Volume 94, Issue 20, Pages 7442-7448Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.2c01373
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Funding
- National Natural Science Foundation of China [21874022, 21675029]
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This study presents a portable photo-electrochemical biosensing technology for the detection of human papillomavirus-16. The technology combines CRISPR-Cas12a with In2O3-In2S3/SPE for biorecognition and photocurrent response, enabling rapid screening of HPV-16 concentration via a smartphone.
This work presented a point-of-care (POC) photo-electrochemical (PEC) biosensing for the detection of human papillomavirus-16 (HPV-16) on a portable electrochemical detection system by using CRISPR-Cas12a trans-cleaving the G-quadruplex for the biorecognition/amplification and a hollow In2O3-In2S3-modified screen-printed electrode (In2O3-In2S3/SPE) as the photoactive material. G-quadruplexes were capable of biocatalytic precipitation (H2O2-mediated 4-chloro-1-naphthol oxidation) on the In2O3-In2S3/SPE surface, resulting in a weakened photocurrent, but suffered from transcleavage when the CRISPR-Cas12a system specifically recognized the analyte. The photocurrent results could be directly observed with the card-sized electrochemical device via a smartphone, which displayed a high-value photocurrent for these positive samples, while a low-value photocurrent for the target-free samples. Such a system exhibited satisfying photocurrent responses toward HPV-16 within a wide working range from 5.0 to 5000 pM and allowed for detection of HPV-16 at a concentration as low as 1.2 pM. The proposed assay provided a smartphone signal readout to enable the rapid screening PEC determination of HPV-16 concentration without sophisticated instruments, thus meeting the requirements of remote areas and resource-limited settings. We envision that combining an efficient biometric PEC sensing platform with a wireless card-sized electrochemical device will enable high-throughput POC diagnostic analysis.
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