Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 414, Issue 11, Pages 3541-3549Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-022-03984-x
Keywords
LC; MS; Catecholamines; Sample preparation; Magnetic bead extraction; Plasma
Funding
- National Key Research and Development Program of China [2021YFC2401100]
- Innovation Led Industrial Cluster Project of Zheng-Luo-Xin National Innovation Demonstration Area [201200211100]
- Beijing Natural Science Foundation [7212087]
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The study established an automated method based on the magnetic bead extraction principle for measuring metanephrines and 3-methoxytyramine in plasma, showing excellent linearity and reproducibility compared to traditional solid-phase extraction. This automated approach may indicate a new era for LC-MS/MS in clinical application.
Liquid chromatography tandem mass spectrometry (LC-MS/MS) is used routinely in clinical diagnostics; however, automating the sample pretreatment is challenging. We established and evaluated an automated method based on the magnetic bead extraction principle (MBE) to measure normetanephrine (NMN), metanephrine (MN), and 3-methoxytyramine (3-MT). The target analytes were extracted, purified, and concentrated using different solvents and chemical bond-modified magnetic beads transferred via a magnetic bar. The linearity, recovery, matrix effect, and precision of MBE were evaluated thoroughly, and compared with traditional solid-phase extraction (SPE) using 131 plasma samples. The chromatography peaks of metanephrines and 3-MT, extracted via MBE, are symmetrical, without interfering peaks. The linearity was excellent with correlation coefficient (r) > 0.99. The MBE exhibited good reproducibility with within-run coefficient variations (CVs) of 1.96-2.00%, 4.06-5.75%, and 3.89-4.90% for MN, NMN, and 3-MT, respectively. The total CVs for MN, NMN, and 3-MT were 1.96-2.80%, 5.12-5.75%, and 5.44-6.27%, respectively. The relative recoveries for MN, NMN, and 3-MT varied between 93.5 and 107.4%, whereas their biases were all within 10%. The results for MN, NMN, and 3-MT extracted via MBE compared with SPE exhibited excellent correlation, with r > 0.99; the mean bias% for MN, NMN, and 3-MT were small (-2.9%, -3.2%, and -3.2%, respectively). In conclusion, the automated MBE method for measuring plasma metanephrines and 3-MT can be applied in future routine clinical diagnostics, and the MBE principle may indicate a new era for LC-MS/MS in clinical application.
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