Target-manipulated drawstring DNAzyme for ultrasensitive detection of UDG using Au@Ag NRs indicator

Article Chemistry, Analytical

Highly Sensitive Detection of miR-21 through Target-Activated Catalytic Hairpin Assembly of X-Shaped DNA Nanostructures

Shui Qin Chai et al.

Summary: The study introduces a simple dual-amplification strategy for highly sensitive miRNA detection, utilizing CHA and multiple fluorophores on an X-shaped DNA nanostructure.

ANALYTICAL CHEMISTRY (2021)

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Article Biophysics

Terminal deoxynucleotidyl transferase combined CRISPR-Cas12a amplification strategy for ultrasensitive detection of uracil-DNA glycosylase with zero background

Yi-Chen Du et al.

Summary: A highly sensitive biosensing strategy utilizing TdT and CRISPR-Cas12a for signal amplification was reported, showing great potential in the design of various biosensors and achieving ultrasensitive detection of UDG and T4 PNK. The biosensors developed based on this strategy display high sensitivity, signal amplification efficiency, and low background noise, making them promising for clinical diagnosis and biomedical research.

BIOSENSORS & BIOELECTRONICS (2021)

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Article Biophysics

Sensitive electrochemical biosensor for Uracil-DNA glycosylase detection based on self-linkable hollow Mn/Ni layered doubled hydroxides as oxidase-like nanozyme for cascade signal amplification

Tingting Liu et al.

Summary: This study presents a sensitive electrochemical biosensor for detecting uracil-DNA glycosylase (UDG) based on hollow Mn/Ni layered doubled hydroxides (h-Mn/Ni LDHs) as oxidase-like nanozyme. By utilizing a strategy of specific excision of uracil and signal amplification without the need for H2O2, the biosensor exhibited excellent stability and performance, highlighting the potential of h-Mn/Ni LDHs for future applications in biological analysis and clinical diagnosis.

BIOSENSORS & BIOELECTRONICS (2021)

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Article Chemistry, Analytical

One-step G-quadruplex-based fluorescence resonance energy transfer sensing method for ratiometric detection of uracil-DNA glycosylase activity

Hengzhi Zhao et al.

Summary: A facile and ratiometric strategy for detecting UDG activity using FRET was developed in this study, which can detect UDG activity and screen UDG inhibitors without requiring additional signal amplification. By measuring system fluorescence intensity and exploiting FRET signal difference, the detection limit is 0.087 U/mL.

TALANTA (2021)

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Article Chemistry, Analytical

The dumbbell probe mediated triple cascade signal amplification strategy for sensitive and specific detection of uracil DNA glycosylase activity

Yayun Yang et al.

Summary: In this study, a dumbbell probe-based triple cascade signal amplification strategy was proposed for sensitive and specific detection of UDG activity. The method significantly improved sensitivity, ensured specificity, and could also be used for inhibitor screening.

TALANTA (2021)

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Article Chemistry, Analytical

Simple Mix-and-Read Assay with Multiple Cyclic Enzymatic Repairing Amplification for Rapid and Sensitive Detection of DNA Glycosylase

Juan Hu et al.

Summary: A simple mix-and-read assay was developed for the sensitive detection of DNA glycosylase using multiple cyclic enzymatic repairing amplification, enabling rapid measurement of hOGG1 without the need for extra primers, and applicable for clinical diagnosis and drug discovery.

ANALYTICAL CHEMISTRY (2021)

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Article Chemistry, Multidisciplinary

Stimuli-Induced Upgrade of Nuclease-Resistant DNA Nanostructure Composed of a Single Molecular Beacon for Detecting Mutant Genes

Chang Xue et al.

Summary: ctDNAs, released from tumor cells and recognized as promising biomarkers for early cancer diagnosis, can be robustly and accurately detected using a novel DNA nanostructure probe. This probe, without the need for auxiliary probes, has a detection limit of 50 fM, can discriminate single nucleotide polymorphisms, and has a wide quantitative range.

ACS SENSORS (2021)

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Article Chemistry, Analytical

Accurate and Nonpurified Identification of Extracellular Vesicles Using Dual-Binding Recognition Mode

Wenyu Sun et al.

Summary: A novel double-positive EV membrane protein recognition mode was developed for accurate identification of EVs, showing excellent specificity and sensitivity. The approach integrates toehold activation and DNAzyme-catalyzed etching of nanorods, allowing for non-purified, low-cost, and visual EV identification.

ANALYTICAL CHEMISTRY (2021)

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Article Chemistry, Analytical

Ag/TiO2 nanocomposites as a novel SERS substrate for construction of sensitive biosensor

Siqi Huang et al.

Summary: The Ag/TiO2 nanocomposites were prepared via catalytic hairpin assembly (CHA) strategy to fabricate a SERS biosensor for sensitive detection of UDG, with the introduction of Pb2+ assisted DNAzyme cleavage reaction enhancing the sensitivity of the method, achieving a detection limit of 1.2×10-5 U mL-1.

SENSORS AND ACTUATORS B-CHEMICAL (2021)

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Article Chemistry, Analytical

A DNAzyme-driven random biped DNA walking nanomachine for sensitive detection of uracil-DNA glycosylase activity

Li Tan et al.

Summary: The study developed a highly specific and ultrasensitive detection method for UDG activity using a DNAzyme-based nanomachine, achieving a detection limit as low as 3.69 x 10(-6) U mL(-1). The method is highly specific to UDG and can be used for specific detection in complex samples, showing potential application prospects in biomedical research and clinical diagnosis related to UDG.

ANALYST (2021)

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Article Biophysics