Journal
ANALYTICA CHIMICA ACTA
Volume 1219, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.aca.2022.340027
Keywords
Exosomes; Split-aptamer; Temperature-sensitive; Regenerable; Electrochemical biosensor
Categories
Funding
- National Natural Science Foundation of China [21804120, 21974125]
- 111 Project of Henan Province [CXJD2021001]
- Program for Innovative Research Team (in Science and Technology) in University of Henan Province [22TRTSTHN002]
- Collaborative Innovation Project of Zhengzhou (Zhengzhou University) [18XTZX12002]
Ask authors/readers for more resources
In this study, a split-aptamer mediated regenerable temperature-sensitive electrochemical biosensor was developed for the detection of exosomes. The sensor allowed sensitive and specific analysis of target exosomes and could be quickly regenerated for reuse.
In this paper, a split-aptamer mediated regenerable temperature-sensitive (SMRT) electrochemical biosensor was constructed for the detection of exosomes. The split-aptamer used in this SMRT biosensor was composed of two fragments, one of which was immobilized on the surface of an electrode via sulfhydryl groups and named split-a and the other was labelled with methylene blue and named split-b. The two fragments could form sandwich structures at the electrode surface via target-induced self-assembly in the presence of target exosomes at 4 degrees C in PBS, and then realizing the detection of exosomes via voltammetry. In addition, due to the temperature sensitivity of the split-aptamer, the electrode could be regenerated through temperature-induced disassembly of the sandwich structures. Consequently, the SMRT biosensor realized sensitive and specific analysis of target exosomes with a limit of detection of 1.5 x 106 particles/mL and could be quickly and easily regenerated by washing with PBS at 37 degrees C for 30 s without any additives. This is the first study on the construction of a reproducible electrochemical biosensor using a split-aptamer for the specific detection of tumour exosomes, and may provide an innovative strategy for the economical and efficient design of regenerable electrochemical biosensors.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available