4.4 Article

Overexpression of recombinant proteins containing non-canonical amino acids in Vibrio natriegens: p-azido-L-phenylalanine as coupling site for 19F-tags

Journal

AMINO ACIDS
Volume 54, Issue 7, Pages 1041-1053

Publisher

SPRINGER WIEN
DOI: 10.1007/s00726-022-03148-2

Keywords

Unnatural amino acids; Orthogonal tRNA; tRNA synthetase; Stop codon suppression; F-19-NMR

Funding

  1. University of Graz
  2. Austrian Science Fund (FWF) [DOC 50]
  3. Land Steiermark
  4. City of Graz
  5. Excellence Initiative BioHealth of the University of Graz

Ask authors/readers for more resources

Vibrio natriegens, known as the fastest growing organism, has potential as an alternative to E. coli in recombinant protein production. In this study, the engineered V. natriegens strain was used to incorporate the non-canonical amino acid AzF into recombinant proteins, resulting in efficient suppression of stop codons and high protein yields. The newly introduced azido-group was used as a coupling site for NMR-sensitive F-19 tags, enabling the observation of protein-observed F-19-NMR.
Vibrio natriegens is the fastest growing organism identified so far. The minimum doubling time of only 9.4 min, the ability to utilize over 60 different carbon sources and its non-pathogenic properties make it an interesting alternative to E. coli as a new production host for recombinant proteins. We investigated the ability of the engineered V. natriegens strain, Vmax (TM) Express, to incorporate the non-canonical amino acid (ncAA) p-azido-L-phenylalanine (AzF) into recombinant proteins for NMR applications. AzF was incorporated into enhanced yellow fluorescent protein (EYFP) and MlaC, an intermembrane transport protein, by stop codon suppression. AzF incorporation into EYFP resulted in an improved suppression efficiency (SE) of up to 35.5 +/- 0.8% and a protein titer of 26.7 +/- 0.7 mg/L. The expression levels of MlaC-AzF even exceeded those of E. coli BL21 cells. For the recording of H-1-N-15 and F-19 NMR spectra, EYFP-AzF was expressed and isotopically labeled in minimal medium and the newly introduced azido-group was used as coupling site for NMR sensitive F-19-tags. Our findings show that Vmax is a flexible expression host, suitable for the incorporation of ncAAs in recombinant proteins with the potential to surpass protein yields of E. coli. The presented method suggests the implementation of V. natriegens for expression of isotopically labeled proteins containing ncAAs, which can be chemically modified for the application in protein-observed F-19-NMR.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.4
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available