Journal
ADVANCED MATERIALS
Volume 34, Issue 27, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adma.202200687
Keywords
deep UV lithography; micropatterning; microstructures; polystyrene; tissue culture
Categories
Funding
- Dutch Province of Limburg
- Stichting De Weijerhorst
- Netherlands Organization for Scientific Research [024.003.013]
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This article presents a simple and accessible method for fabricating microstructures in polystyrene to guide cell behavior.
Tissue-culture-ware polystyrene is the gold standard for in vitro cell culture. While microengineering techniques can create advanced cell microenvironments in polystyrene, they require specialized equipment and reagents, which hinder their accessibility for most biological researchers. An economical and easily accessible method is developed and validated for fabricating microstructures directly in polystyrene with sizes approaching subcellular dimensions while requiring minimal processing time. The process involves deep ultraviolet irradiation through a shadow mask or ink pattern using inexpensive, handheld devices followed by selective chemical development with common reagents to generate micropatterns with depths/heights between 5 and 10 mu m, which can be used to guide cell behavior. The remarkable straightforwardness of the process enables this class of microengineering techniques to be broadly accessible to diverse research communities.
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