Journal
ADVANCED FUNCTIONAL MATERIALS
Volume 32, Issue 27, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adfm.202202020
Keywords
degradable; oxygen; sensors; silk; subcutaneous
Ask authors/readers for more resources
Silk-protein biomaterials can be used as scaffold materials in oxygen sensors, providing excellent stability and biocompatibility. Silk films containing oxygen-sensing chromophores show good sensing functionality in vitro and in vivo, operating in various physiological states.
Continuous monitoring of biochemical analytes like oxygen is of interest in biomedicine to provide insight into physiology and health. Silk-protein biomaterials are particularly useful as the scaffold material in oxygen sensors due to silk's unique amphiphilic chemistry, which promotes noncovalent stabilization of the protein and additives in aqueous environments. Silk films containing a water-insoluble oxygen-sensing chromophore, Pd (II) tetramethacrylated benzoporphyrin (PdBMAP), are evaluated as optical oxygen sensors in vitro and in vivo. These silk-chromophore composites are stabilized by the self-assembled, physically crosslinked protein network. The deaerated phosphorescence lifetime (tau(m,0) approximate to 300 mu s) of the chromophore in vitro is quenched to 50% of its initial value at approximate to 31 mu m dissolved oxygen, indicating sensing functionality within physiological ranges of oxygen. In vitro enzymatic degradation of the silk films with and without the chromophore is demonstrated. The silk-chromophore composite films are cytocompatible in vitro, biocompatible in vivo upon implantation in rats, and displayed mechanical properties suitable for subcutaneous implantation. Further, the films maintain oxygen-sensing function in vivo and demonstrate real-time sensing capabilities throughout various physiological states (i.e., hyperoxia, normoxia, and hypoxia).
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available