Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 14, Issue 23, Pages 26548-26556Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.2c07317
Keywords
extracellular vesicles; plasmonic sensing; surface chemistry; antibody immobilization; physisorption
Funding
- National Institute of Health [R21CA217662, R01GM138778]
- Technology Innovation Program - Ministry of Trade, Industry and Energy, Republic of Korea [20008829]
- Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI) - Ministry of Health & Welfare, Republic of Korea [HI21C0957]
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education [NRF-2021R1A6A3A14039686]
- Korea Evaluation Institute of Industrial Technology (KEIT) [20008829] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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This study reports a method for the direct immobilization of antibodies on plasmonic gold surfaces via physisorption, which shows excellent capture of cancer-derived EVs with ultralow non-specific binding. Compared to commonly used methods involving linker attachment and chemical reactions, this method demonstrates higher specific capture rate and significantly lower non-specific binding. The method provides a simple, fast, and reproducible means for functionalizing plasmonic gold surfaces with antibodies for robust EV biosensing.
Plasmonic biosensors are increasingly being used for the analysis of extracellular vesicles (EVs) originating from disease areas. However, the high non-specific binding of EVs to a gold-sensing surface has been a critical problem and hindered the true translational potential. Here, we report that direct antibody immobilization on the plasmonic gold surface via physisorption shows excellent capture of cancer-derived EVs with ultralow non-specific binding even at very high concentrations. Contrary to commonly used methods that involve thiol-based linker attachment and an EDC/sulfo-NHS reaction, we show a higher specific capture rate and >50-fold lower non-specific on citrate-capped plain and nanopatterned gold surfaces. The method provides a simple, fast, and reproducible means to functionalize plasmonic gold surfaces with antibodies for robust EV biosensing.
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