4.8 Article

Designing Electrochemical Biosensing Platforms Using Layered Carbon-Stabilized Porous Silicon Nanostructures

Journal

ACS APPLIED MATERIALS & INTERFACES
Volume 14, Issue 13, Pages 15565-15575

Publisher

AMER CHEMICAL SOC
DOI: 10.1021/acsami.2c02113

Keywords

porous silicon; layered nanostructures; controllable surface chemistry; dual-surface functionality; electrochemical biosensor

Funding

  1. Australian Research Council [DP160104362, LP160101050]
  2. US National Science Foundation (NSF) through the UC San Diego Materials Research Science and Engineering Center (UCSD MRSEC) [DMR-2011924]
  3. NSF through the UC San Diego Materials Research Science and Engineering Center (UCSD MRSEC) [DMR-2011924]
  4. National Science Foundation [ECCS1542148]
  5. National Health and Medical Research Council (NHMRC) of Australia [GNT1125400]
  6. CSIRO
  7. Australian Research Council [LP160101050] Funding Source: Australian Research Council

Ask authors/readers for more resources

This study explores layered carbon-stabilized pSi nanostructures as an electrochemical biosensor, demonstrating its excellent electrochemical properties and high sensitivity for DNA detection. The biosensor achieves a detection limit of 0.4 pM for a 28-nucleotide DNA sequence, two orders of magnitude lower than any other pSi-based electrochemical DNA sensors reported to date.
Porous silicon (pSi) is an established porous material that offers ample opportunities for biosensor design thanks to its tunable structure, versatile surface chemistry, and large surface area. Nonetheless, its potential for electrochemical sensing is relatively unexplored. This study investigates layered carbon-stabilized pSi nanostructures with site-specific functionalities as an electrochemical biosensor. A double-layer nanostructure combining a top hydrophilic layer of thermally carbonized pSi (TCpSi) and a bottom hydrophobic layer of thermally hydrocarbonized pSi (THCpSi) is prepared. The modified layers are formed in a stepwise process, involving first an electrochemical anodization step to generate a porous layer with precisely defined pore morphological features, followed by deposition of a thin thermally carbonized coating on the pore walls via temperature-controlled acetylene decomposition. The second layer is then generated beneath the first by following the same two-step process, but the acetylene decomposition conditions are adjusted to deposit a thermally hydrocarbonized coating. The double-layer platform features excellent electrochemical properties such as fast electron-transfer kinetics, which underpin the performance of a TCpSi-THCpSi voltammetric DNA sensor. The biosensor targets a 28-nucleotide single-stranded DNA sequence with a detection limit of 0.4 pM, two orders of magnitude lower than the values reported to date by any other pSi-based electrochemical DNA sensor.

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