Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 14, Issue 11, Pages 13070-13078Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.2c00376
Keywords
light-activated; gold nanoparticles; miRNA; triple-helix; signal amplification
Funding
- National Natural Science Foundation of China [22076073, 21775063]
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In this study, a light-activated nanodevice based on DNA nanotechnology was constructed for high precision detection of low-abundance miRNA. The nanodevice enabled on-demand imaging of miRNA in living cells with high spatial and temporal control.
Low-abundance biomarker amplification detection systems have been widely used to detect miRNAs; however, always active systems are insufficient for high spatial and temporal control of miRNAs. Here, we constructed a light-activated nanodevice (LAN) based on DNA nanotechnology for high spatial and temporal precision detection of low-abundance miRNA. Light-activated hairpin probes and triple-helix molecular switches were modified on the surface of gold nanoparticles (AuNPs) to trigger miRNA on-demand imaging analysis by UV light activation. In the presence of both UV light and miRNA, the LAN releases hairpin DNA and completes the hybridization chain reaction (HCR) with the conformation-altered triple-helix molecular switch, enabling fluorescence imaging of low-abundance miRNAs in living cells. The current work provides an opportunity to develop light-activated signal amplification sensors that can accurately image miRNAs on-demand in both temporal and spatial dimensions.
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