Journal
CCS CHEMISTRY
Volume 4, Issue 1, Pages 356-368Publisher
CHINESE CHEMICAL SOC
DOI: 10.31635/ccschem.021.202000679
Keywords
luminescent probes; liver injury and repair; fluorescence imaging; peroxynitrite; GSH
Categories
Funding
- NSFC [21877029, 22074036, 21735001]
- National Key R&D Program of China [2019YFA0210100]
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This study proposes a rational design strategy for a reversible upconversion luminescence nanoprobe for real-time and in vivo imaging of liver injury and repair processes. The novel nanoprobe successfully monitors and differentiates the various degrees of liver injury and repair in a mouse model.
As the major organ for drug metabolism and detoxification, the liver is prone to damage and severely impaired functionality. The treatment of liver diseases is based on a clear understanding of the process underlying liver injury and repair. However, intravital real-time imaging of liver injury and repair is still limited due to the lack of in vivo reversible visualization methods. To this end, we proposed a rational design strategy for the development of a reversible upconversion luminescence nanoprobe that allows real-time and in vivo imaging of liver injury and repair processes. As a proof of concept, we first developed a small molecule probe NB3 which can reversibly respond to related analytes of early liver injury [peroxynitrite (ONOO-)] and liver repair [glutathione (GSH)]. The small molecule probe was then integrated with a core-shell upconversion nanoparticle to form a sophisticated nanoprobe. Compared with traditional small molecule probes, this nanoprobe exhibited a higher selectivity to ONOO-, longer retention time in liver, and wider dynamic response range to GSH after oxidation by ONOO-. The novel nanoprobe facilitated the successful monitoring and discrimination among the different degrees of liver injury and repair in a mouse model. [GRAPHICS] .
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