Streptomyces verrucosisporus sp nov., isolated from marine sediments

Five actinomycete isolates, CPB1-1(T), CPB2-10, BM1-4, CPB3-1 and CPB1-18, belonging to the genus Streptomyces were isolated from marine sediments collected from Chumphon Province, Thailand. They produced open loops of warty spore chains on aerial mycelia. LL-Diaminopimelic acid, glucose and ribose were found in their whole-cell hydrolysates. Polar lipids found were diphosphatidylglycerol, phosphatidylethanolamine, lysophosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside. Menaquinones were MK-9(H-6), MK-9(H-8), MK-10(H-6) and MK-10(H-8). Major cellular fatty acids were anteisoC(15:0), anteiso-C-17:0 and iso-C-16:0. The taxonomic position of the strains was described using a polyphasic approach. BLASTN analysis of the 16S rRNA gene sequence revealed that these five strains exhibited the highest similarities with 'Streptomyces mangrovicola' GY1 (99.0 %), Streptomyces fenghuangensis GIMN4.003(T) (98.6 %), Streptomyces barkulensis RC 1831(T) (98.5 %) and Streptomyces radiopugnans R97(T) (98.3 %). However, their phenotypic characteristics and 16S rRNA gene sequences as well as DNA-DNA relatedness differentiated these five strains from the other species of the genus Streptomyces. Here, we propose the novel actinomycetes all being representatives of the same novel species, Streptomyces verrucosisporus, with type strain CPB1-1(T) (= JCM 18519(T) = PCU 343(T) = TISTR 2344(T)).