Methyloterrigena soli gen. nov., sp nov., a methanol-utilizing bacterium isolated from chloroethylene-contaminated soil

A Gram-stain-negative, aerobic, short rod-shaped and motile bacterial strain, designated M48(T), was isolated from a soil sample contaminated with chloroethylene compounds. Strain M48(T) was able to biodegrade methanol as a sole carbon source. Based on 16S rRNA gene sequences, strain M48(T) was closely related to Devosia chinhatensis IPL18(T), Devosia insulae DS-56(T), Vasilyevaea enhydra 9b(T) and Devosia psychrophila Cr7-05(T), showing sequence similarities of 96.0, 95.8, 95.6 and 95.5 ok, respectively. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain M48(T) formed a distinct lineage within the family Hyphomicrobiaceae of the class Alphaproteobacteria. Strain M48(T) contains Q-10 as the predominant ubiquinone, and C-18:1 omega 7c 11-metyl, C-16:0, C-19:0 cyclo omega 8c and C-18:1 omega 7c as the major fatty acids. The polar lipid profile includes diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and dimannosyldiacylglycerol. The DNA G + C content of strain M48(T) was 60.3 mol%. Based on phenotypic, phylogenetic and chemotaxonomic data, strain M48(T) represents a novel species of a new genus within the family Hyphomicrobiaceae, for which the name Methyloterrigena soli gen. nov., sp. nov. is proposed. The type strain of the type species is M48(T) (=KEMB 224-262(T)=JCM 30821(T)).