Marinibaculum pumilum gen. nov., sp nov., isolated from seawater

A Gram-stain-negative, facultatively anaerobic, motile and rod-shaped strain, designed H2(T), was isolated from the Western Pacific Ocean, and subjected to a taxonomic investigation using a polyphasic approach. Strain H2(T) grew at 15-40 degrees C and pH 6.0-9.0 (optimum 37 degrees C and pH 6.5), and with 1-10% (w/v) NaCI (optimum 2%). The predominant respiratory quinone was ubiquinone-10 (Q-10) and the major fatty acids identified were C-19:0 CYCIO omega 8C, C-18 :1 omega 7c, C-18:0 and 11-methyl-C-18:1 omega 7c. The polar lipids of strain H2(T) consisted of phosphatidylglycerol, one unknown phospholipid, one unknown glycolipid and three unidentified aminolipids. The DNA G+C content was 75.0 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain H2(T) formed a distinct Glade belong to the family Rhodospirillaceae within the Alphaproteobacteria. On the basis of morphological, physiological and chemotaxonomic characteristics, together with the results of phylogenetic analysis, strain H2(T) represents a novel species in a new genus in the family Rhodospirillaceae, for which the name Marinibaculum pumilum gen. nov., sp. nov. is proposed. The type strain of the type species is H2(T)(=MCCC 1K02279(T)=KCTC 42964(T)).