Paenibacillus solani sp nov., isolated from potato rhizosphere soil

A novel Gram-stain-positive, endospore-forming bacterium, designated FJAT-22460(T), was isolated from a soil sample of a potato field in Xinjiang Autonomous Region, China. Cells were rods that were catalase-positive and motile by peritrichous flagella. The strain was found to grow at temperatures ranging from 10 to 40 degrees C (optimum 30 degrees C) and at pH 5.0-12.0 (optimum pH 7) with 0-5% (w/v) NaCl (optimum 0% NaCl). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain FJAT-22460(T) belonged to the genus Paenibacillus and exhibited 16S rRNA gene sequence similarities of 97.3, 97.2, 97.2 and 97.0% with Paenibacillus glucanolyticus DSM 5162(T),Paenibacillus lautus DSM 3035(T), Paenibacillus lactis MB 1871(T) and Paenibacillus chibensis JCM 9905(T), respectively. DNA-DNA relatedness of strain FJAT-22460(T) with Paenibacillus glucanolyticus DSM 5162(T) and Paenibacillus lautus DSM 3035(T) was 62.6% and 33.3%, respectively, lower than the 70% accepted for species delineation. The menaquinone was identified as MK-7. The major fatty acids detected were anteiso-C-15:0 (51.4 %), iso-C-15 : 0 (5.3 %), C-16 : 0 (12.1 %), iso-C-16 : 0 (10.7 %) and anteiso-C-17 : 0 (6.9 %). The DNA G+C content was determined to be 50.9 mol%. Phenotypic, chemotaxonomic and genotypic properties clearly indicated that isolate FJAT-22460(T) represents a novel species within the genus Paenibacillus, for which the name Paenibacillus solani sp. nov. is proposed. The type strain is FJAT-22460T (=DSM 100999(T) =CCTCC AB 2015207(T)).