Arvibacter flaviflagrans gen. nov., sp nov., isolated from forest soil

During a study of the bacterial community in forest soil of Kyonggi University, Suwon, South Korea, a glistening yellow-pigmented, Gram-stain-negative, aerobic, motile and rod-shaped bacterium, designated strain C-1-16(T), was isolated. This strain was non-sporulating, catalase-negative and oxidase-positive. It was able to grow at 20-42 degrees C, at pH 6.0-9.0 and with 0-0.5% (w/v) NaCl. This strain was taxonomically characterized by a polyphasic approach. Based on the results of 16S rRNA gene sequence analysis, strain C-1-16(T) formed a lineage within the family Chitinophagaceae of the phylum Bacteroidetes and closely related to the genera Filimonas (93.53% sequence similarity), Sediminibacterium (92.52-90.75 %), Lacibacter (91.99-91.19 %) and Parasegetibacter (91.88-91.78 %). Flexirubin-type pigment was present for strain C-1-16(T). The only respiratory quinone was menaquinone-7 (MK-7) and the major polar lipid was phosphatidylethanolamine. The predominant fatty acids of strain C-1-16(T) were iso-C-15 : 0, iso-C-17 (: 0) 3-OH, iso-C-15 : 1 G, summed feature 3 (C-16 : 1 omega 6c and/or C-16 : 1 omega 7c) and C-16 : 0. The genomic DNA G+C content of this novel strain was 45.5 mol%. On the basis of phenotypic, genotypic and phylogenetic analysis, strain C-1-16(T) represents a novel species of a new genus in the family Chitinophagaceae, for which the name Arvibacter flaviflagrans gen. nov., sp. nov. is proposed. The type strain of Arvibacter flaviflagrans is C-1-16(T) (= KEMB 900-374(T) = KACC 18717(T) = JCM 31293(T)).