Sellimonas intestinalis gen. nov., sp nov., isolated from human faeces

A Gram-stain-positive and obligately anaerobic bacterial strain, BR72(T), forming ivory yellow colonies was isolated from a faecal sample of a healthy Korean woman. 16S rRNA gene sequence analysis indicated that strain BR72(T) belongs to Clostridium cluster XIVa and represents a distinct phyletic line within the family Lachnospiraceae. The most closely related strains were Clostridium nexile DSM 1787(T) (94.1 % 16S rRNA gene sequence similarity), Coprococcus comes ATCC 27758(T) (93.5 %), Ruminococcus torques ATCC 27756(T) (93.5 %), Ruminococcus lactaris ATCC 29176(T) (93.5 %), Clostridium aerotolerans DSM 5434(T) (93.1 %) and Eubacterium fissicatena DSM 3598(T) (92.9 %). The DNA G+C content of strain BR72(T) based on its genome sequence was 45.3 mol%. The major cellular fatty acids were C16 : 0, C-14 : 0, and iso-C-17 : 1 I and/or anteiso-C-17 : 1 B. Acetic acid was produced from glucose fermentation. Other physiological and biochemical comparisons allowed the phenotypic differentiation of strain BR72(T) from the members of the family Lachnospiraceae. Based on the phylogenetic and phenotypic findings, this strain is considered to represent a novel species of a new genus belonging to the family Lachnospiraceae and the name Sellimonas intestinalis gen. nov., sp. nov. is proposed. The type strain of Sellimonas intestinalis is BR72(T) (=KCTC 15479(T)=JCM 30749(T)).