Terasakiella brassicae sp nov., isolated from the wastewater of a pickle-processing factory, and emended descriptions of Terasakiella pusilla and the genus Terasakiella

A Gram-stain-negative, motile, polyhydroxybutyrate-accumulating, aerobic, S-shaped bacterium, designated B3(T), was isolated from the wastewater of a pickle-processing factory. 16S rRNA gene sequence similarity analysis showed that it was most closely related to the type strain, Terasakiella pusilla (96.6 % similarity). Strain B3(T) was able to grow at 4-40 degrees C (optimum 32-37 degrees C), pH 5.5-9.0 (optimum 6.5-7.5) and with 0.5-8 % (w/v) NaCl present (optimum 1-2 %, w/v). Chemotaxonomic analysis showed that the respiratory quinone was ubiquinone Q-10, the major fatty acids included C-16 : 0, C-18 : 1 omega 7c and C-16 : 1 omega 7c and/or iso-C-15: 2-OH. The major polar lipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylserine, aminophospholipid and three uncharacterized phospholipids. The genomic DNA G+ C content of strain B3(T) was 42.3 mol%. The DNA-DNA relatedness value between B3(T) and T. pusilla DSM 9263(T) was 23.9 %. On the basis of the phenotypic, chemotaxonomic and genotypic characteristics of strain B3(T), it represents a novel species of the genus Terasakiella, for which the name Terasakiella brassicae sp. nov. is proposed. The type strain is B3(T) (=KCTC 42652(T) =CGMCC 1.15254(T)). Emended descriptions of T. pusilla and the genus Terasakiella are also presented.