Woeseia oceani gen. nov., sp nov., a chemoheterotrophic member of the order Chromatiales, and proposal of Woeseiaceae fam. nov.

A novel Gram-stain-negative, rods or bent rods, facultatively anaerobic, oxidase-negative and catalase-positive bacterium, designated XK5(T), was isolated from coastal sediment from Xiaoshi Island, Weihai, China. Optimal growth occurred at 28-35 degrees C (range 8-42 degrees C) and pH 7.0-8.0 (range pH 6.0-9.0) with 1-3 % (w/v) NaCI (range 0.5-8 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain XK5(T) was 92.1 % similar to the type strain of Thioalkalivibrio thiocyanodenitrificans, 91.9 % to the type strain of Thioalkalivibrio sulfidiphilus and 91.8 % to the type strain of Thioalkalivibrio denitrificans; similarity to other species was less than 91 %. The isolate and closely related environmental clones formed a novel family level Glade in the order Chromatiales. The polar lipid profile of the novel isolate consisted of phosphatidylethanolamine, phosphatidylglycerol and some other unknown phospholipids, aminolipids and lipids. Major cellular fatty acids were iso-C-17:1 omega 9c and iso-C-15:0 and the main respiratory lipoquinone was Q-8. The DNA G+C content of strain XK5(T) was 59.3 mol%. Comparative analysis of 16S rRNA gene sequences and characterization indicated that strain XK5(T) represents a novel species of a new genus within a novel family of the order Chromatiales, for which the name Woeseia oceani gen. nov., sp. nov. is proposed. The type strain of Woeseia oceani is XK5(T) (=ATCC BAA-2615(T)=CICC 10905(T)). In addition, a novel family name, Woeseiaceae fam. nov., is proposed to accommodate the genus Woeseia.